Generation of loss-of-function mutants for wheat rust disease resistance gene cloning

Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, Evans Lagudah

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

One of the most important tools to identify and validate rust resistance gene function is by producing lossof- function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.
Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.humana@humanapr.com
Pages199-205
Number of pages7
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-20

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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