Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Ruizhu Zeng; Harwin Sidik; Kim S. Robinson; Franklin L. Zhong; Bruno Reversade; Mahmoud A. Pouladi

doi:10.1016/j.scr.2019.101533

Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Ruizhu Zeng, Harwin Sidik, Kim S. Robinson, Franklin L. Zhong, Bruno Reversade, Mahmoud A. Pouladi

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

Original language	English (US)
Journal	Stem Cell Research
Volume	40
DOIs	https://doi.org/10.1016/j.scr.2019.101533
State	Published - Oct 1 2019
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-02-15

Access to Document

10.1016/j.scr.2019.101533

Cite this

@article{80677c80331e4721b27d043e6af437a7,

title = "Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2",

abstract = "Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.",

author = "Ruizhu Zeng and Harwin Sidik and Robinson, {Kim S.} and Zhong, {Franklin L.} and Bruno Reversade and Pouladi, {Mahmoud A.}",

note = "Generated from Scopus record by KAUST IRTS on 2023-02-15",

year = "2019",

month = oct,

day = "1",

doi = "10.1016/j.scr.2019.101533",

language = "English (US)",

volume = "40",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

AU - Zeng, Ruizhu

AU - Sidik, Harwin

AU - Robinson, Kim S.

AU - Zhong, Franklin L.

AU - Reversade, Bruno

AU - Pouladi, Mahmoud A.

N1 - Generated from Scopus record by KAUST IRTS on 2023-02-15

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

AB - Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

UR - https://linkinghub.elsevier.com/retrieve/pii/S1873506119301631

UR - http://www.scopus.com/inward/record.url?scp=85070998455&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2019.101533

DO - 10.1016/j.scr.2019.101533

M3 - Article

SN - 1873-5061

VL - 40

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this