Functional expression of TrkB receptors on interneurones and pyramidal cells of area CA3 of the rat hippocampus

Ernesto Griego, Gabriel Herrera-López, Gisela Gómez-Lira, Germán Barrionuevo, Rafael Gutiérrez, Emilio J. Galván*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The dentate gyrus and hippocampal area CA3 region of the mammalian brain contains the highest levels of brain-derived neurotrophic factor (BDNF) and its canonical membrane receptor, tropomyosin-related kinase B (TrkB). Therefore, the present study examines the expression and physiological responses triggered by activation of TrkB on hippocampal area CA3 interneurones and pyramidal cells of the rat hippocampus. Triple immunolabelling for TrkB, glutamate decarboxylase 67, and the calcium-binding proteins parvalbumin, calbindin or calretinin confirms the somatic expression of TrkB in all CA3 sublayers. TrkB-positive interneurones with fast-spiking discharge are restricted to strata oriens and lucidum, whereas regular-spiking interneurones are found in the strata lucidum, radiatum and lacunosum-moleculare. Activation of TrkB receptors with 7,8-dihydroxyflavone (DHF) modulates amplitude and frequency of spontaneous synaptic currents recorded from CA3 interneurones. Furthermore, the isolated excitatory postsynaptic currents (EPSC) of CA3 interneurones evoked by the mossy fibres (MF) or commissural/associational (C/A) axons, show input-specific synaptic potentiation in response to TrkB stimulation. On CA3 pyramidal cells, stimulation with DHF potentiates the MF synaptic transmission and increases the MF-EPSP – spike coupling. The latter exhibits a dramatic increase when picrotoxin is bath perfused after DHF, indicating that local interneurones restrain the excitability mediated by activation of TrkB. Therefore, we propose that release of BDNF on area CA3 reshapes the output of this hippocampal region by simultaneous activation of TrkB on GABAergic interneurones and pyramidal cells.

Original languageEnglish (US)
Article number108379
JournalNeuropharmacology
Volume182
DOIs
StatePublished - Jan 2021

Bibliographical note

Funding Information:
This work includes data from Ernesto Griego's Ph.D. dissertation thesis and was supported by Conacyt ( National Council of Science and Technology, Mexico ) grants CB-2016-281617 to EJG and CB-25439 to RG, and fellowship 727269 to Ernesto Griego.

Funding Information:
This work was supported by Consejo Nacional de Ciencia y Tecnolog?a, M?xico grant CB-2016-281617 to EJG, grant CB-254339 to RG and fellowships 727,269 (EG) and 367,017 (GH-L).This work includes data from Ernesto Griego's Ph.D. dissertation thesis and was supported by Conacyt (National Council of Science and Technology, Mexico) grants CB-2016-281617 to EJG and CB-25439 to RG, and fellowship 727269 to Ernesto Griego.

Funding Information:
This work was supported by Consejo Nacional de Ciencia y Tecnología , México grant CB-2016-281617 to EJG , grant CB-254339 to RG and fellowships 727,269 (EG) and 367,017 (GH-L).

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

  • Associational synapses
  • BDNF
  • CA3 interneurones
  • CA3 pyramidal neurones
  • Commissural
  • Mossy fibre synapses
  • TrkB

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Functional expression of TrkB receptors on interneurones and pyramidal cells of area CA3 of the rat hippocampus'. Together they form a unique fingerprint.

Cite this