Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice

Jingxia Shao; Imran Haider; Lizhong Xiong; Xiaoyi Zhu; Rana Muhammad Fraz Hussain; Elin Övernäs; Annemarie H. Meijer; Gaisheng Zhang; Mei Wang; Harro J. Bouwmeester; Pieter B.F. Ouwerkerk

doi:10.1371/journal.pone.0199248

Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice

Jingxia Shao, Imran Haider, Lizhong Xiong, Xiaoyi Zhu, Rana Muhammad Fraz Hussain, Elin Övernäs, Annemarie H. Meijer, Gaisheng Zhang, Mei Wang, Harro J. Bouwmeester, Pieter B.F. Ouwerkerk^*

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.

Original language	English (US)
Article number	e0199248
Journal	PloS one
Volume	13
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0199248
State	Published - Jul 2018

Bibliographical note

Publisher Copyright:
© 2018 Shao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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title = "Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice",

abstract = "The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.",

author = "Jingxia Shao and Imran Haider and Lizhong Xiong and Xiaoyi Zhu and Hussain, {Rana Muhammad Fraz} and Elin {\"O}vern{\"a}s and Meijer, {Annemarie H.} and Gaisheng Zhang and Mei Wang and Bouwmeester, {Harro J.} and Ouwerkerk, {Pieter B.F.}",

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AU - Haider, Imran

AU - Xiong, Lizhong

AU - Zhu, Xiaoyi

AU - Hussain, Rana Muhammad Fraz

AU - Övernäs, Elin

AU - Meijer, Annemarie H.

AU - Zhang, Gaisheng

AU - Wang, Mei

AU - Bouwmeester, Harro J.

AU - Ouwerkerk, Pieter B.F.

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N2 - The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.

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Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice

Abstract

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