Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Moataz Abd El Ghany; Lars Barquist; Simon Clare; Cordelia Brandt; Matthew Mayho; Enrique Joffre´; Åsa Sjöling; A. Keith Turner; John D. Klena; Robert A. Kingsley; Grant A. Hill-Cawthorne; Gordon Dougan; Derek Pickard

doi:10.1099/mgen.0.000554

Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

Moataz Abd El Ghany, Lars Barquist, Simon Clare, Cordelia Brandt, Matthew Mayho, Enrique Joffre´, Åsa Sjöling, A. Keith Turner, John D. Klena, Robert A. Kingsley, Grant A. Hill-Cawthorne, Gordon Dougan, Derek Pickard

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4 Scopus citations

Abstract

Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

Original language	English (US)
Journal	Microbial genomics
Volume	7
Issue number	6
DOIs	https://doi.org/10.1099/mgen.0.000554
State	Published - Jun 10 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-06-16
Acknowledgements: The authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data.

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https://repository.kaust.edu.sa/bitstream/10754/669590/1/mgen000554.pdf

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Abd El Ghany, M., Barquist, L., Clare, S., Brandt, C., Mayho, M., Joffre´, E., Sjöling, Å., Turner, A. K., Klena, J. D., Kingsley, R. A., Hill-Cawthorne, G. A., Dougan, G., & Pickard, D. (2021). Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization. Microbial genomics, 7(6). https://doi.org/10.1099/mgen.0.000554

@article{c8d548a91a1c48cfaf214dbffb35bbda,

title = "Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization",

abstract = "Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.",

author = "{Abd El Ghany}, Moataz and Lars Barquist and Simon Clare and Cordelia Brandt and Matthew Mayho and Enrique Joffre´ and {\AA}sa Sj{\"o}ling and Turner, {A. Keith} and Klena, {John D.} and Kingsley, {Robert A.} and Hill-Cawthorne, {Grant A.} and Gordon Dougan and Derek Pickard",

note = "KAUST Repository Item: Exported on 2021-06-16 Acknowledgements: The authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data.",

year = "2021",

month = jun,

day = "10",

doi = "10.1099/mgen.0.000554",

language = "English (US)",

volume = "7",

journal = "Microbial genomics",

issn = "2057-5858",

publisher = "Microbiology Society",

number = "6",

}

Abd El Ghany, M, Barquist, L, Clare, S, Brandt, C, Mayho, M, Joffre´, E, Sjöling, Å, Turner, AK, Klena, JD, Kingsley, RA, Hill-Cawthorne, GA, Dougan, G & Pickard, D 2021, 'Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization', Microbial genomics, vol. 7, no. 6. https://doi.org/10.1099/mgen.0.000554

TY - JOUR

T1 - Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

AU - Abd El Ghany, Moataz

AU - Barquist, Lars

AU - Clare, Simon

AU - Brandt, Cordelia

AU - Mayho, Matthew

AU - Joffre´, Enrique

AU - Sjöling, Åsa

AU - Turner, A. Keith

AU - Klena, John D.

AU - Kingsley, Robert A.

AU - Hill-Cawthorne, Grant A.

AU - Dougan, Gordon

AU - Pickard, Derek

N1 - KAUST Repository Item: Exported on 2021-06-16 Acknowledgements: The authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data.

PY - 2021/6/10

Y1 - 2021/6/10

N2 - Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

AB - Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.

UR - http://hdl.handle.net/10754/669590

UR - https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000554

U2 - 10.1099/mgen.0.000554

DO - 10.1099/mgen.0.000554

M3 - Article

C2 - 34110281

SN - 2057-5858

VL - 7

JO - Microbial genomics

JF - Microbial genomics

IS - 6

ER -

Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization

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