Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells

Natalia Malara; Maria Laura Coluccio; Tania Limongi; Monica Asande; Valentina Trunzo; Gheorghe Cojoc; Cinzia Raso; Patrizio Candeloro; Gerardo Perozziello; Raffaella Raimondo; Stefania De Vitis; Laura Roveda; Maria Renne; Ubaldo Prati; Vincenzo Mollace; Enzo M. Di Fabrizio

doi:10.1002/smll.201400498

Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells

Natalia Malara, Maria Laura Coluccio, Tania Limongi, Monica Asande, Valentina Trunzo, Gheorghe Cojoc, Cinzia Raso, Patrizio Candeloro, Gerardo Perozziello, Raffaella Raimondo, Stefania De Vitis, Laura Roveda, Maria Renne, Ubaldo Prati, Vincenzo Mollace, Enzo M. Di Fabrizio

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English (US)
Pages (from-to)	n/a-n/a
Number of pages	1
Journal	Small
Volume	10
Issue number	21
DOIs	https://doi.org/10.1002/smll.201400498 https://doi.org/10.1002/smll.201470135
State	Published - Jul 14 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was partially supported by the Grants from the PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the "Fondo Sociale Europeo - POR Calabria FSE 2007/2013" Program. The authors thank R. Giammaria for revising the English text.

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General Medicine

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Malara, N., Coluccio, M. L., Limongi, T., Asande, M., Trunzo, V., Cojoc, G., Raso, C., Candeloro, P., Perozziello, G., Raimondo, R., De Vitis, S., Roveda, L., Renne, M., Prati, U., Mollace, V., & Di Fabrizio, E. M. (2014). Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells. Small, 10(21), n/a-n/a. https://doi.org/10.1002/smll.201400498, https://doi.org/10.1002/smll.201470135

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title = "Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells",

abstract = "Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.{\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

author = "Natalia Malara and Coluccio, {Maria Laura} and Tania Limongi and Monica Asande and Valentina Trunzo and Gheorghe Cojoc and Cinzia Raso and Patrizio Candeloro and Gerardo Perozziello and Raffaella Raimondo and {De Vitis}, Stefania and Laura Roveda and Maria Renne and Ubaldo Prati and Vincenzo Mollace and {Di Fabrizio}, {Enzo M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the Grants from the PON {"}Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti{"} project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB {"}Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet{"} project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the {"}Fondo Sociale Europeo - POR Calabria FSE 2007/2013{"} Program. The authors thank R. Giammaria for revising the English text.",

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Malara, N, Coluccio, ML, Limongi, T, Asande, M, Trunzo, V, Cojoc, G, Raso, C, Candeloro, P, Perozziello, G, Raimondo, R, De Vitis, S, Roveda, L, Renne, M, Prati, U, Mollace, V & Di Fabrizio, EM 2014, 'Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells', Small, vol. 10, no. 21, pp. n/a-n/a. https://doi.org/10.1002/smll.201400498, https://doi.org/10.1002/smll.201470135

TY - JOUR

T1 - Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells

AU - Malara, Natalia

AU - Coluccio, Maria Laura

AU - Limongi, Tania

AU - Asande, Monica

AU - Trunzo, Valentina

AU - Cojoc, Gheorghe

AU - Raso, Cinzia

AU - Candeloro, Patrizio

AU - Perozziello, Gerardo

AU - Raimondo, Raffaella

AU - De Vitis, Stefania

AU - Roveda, Laura

AU - Renne, Maria

AU - Prati, Ubaldo

AU - Mollace, Vincenzo

AU - Di Fabrizio, Enzo M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was partially supported by the Grants from the PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the "Fondo Sociale Europeo - POR Calabria FSE 2007/2013" Program. The authors thank R. Giammaria for revising the English text.

PY - 2014/7/14

Y1 - 2014/7/14

N2 - Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

UR - http://hdl.handle.net/10754/563624

UR - http://doi.wiley.com/10.1002/smll.201400498

UR - http://www.scopus.com/inward/record.url?scp=84928940938&partnerID=8YFLogxK

U2 - 10.1002/smll.201400498

DO - 10.1002/smll.201400498

M3 - Article

SN - 1613-6810

VL - 10

SP - n/a-n/a

JO - Small

JF - Small

IS - 21

ER -

Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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