We introduce theoretically and demonstrate experimentally a method for the determination of three-dimensional absorption anisotropy (three-dimensional linear dichroism) of single luminescent nanoparticles. The anisotropy is measured by monitoring polarization-modulated fluorescence intensity excited alternately by epi-illumination and by total internal reflection. As an example of the application of the method, three-dimensional linear dichroism measured on single light-harvesting systems of photosynthetic bacteria is presented.
ASJC Scopus subject areas
- General Physics and Astronomy
- Physical and Theoretical Chemistry