Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

Marta Sá; Joana Monte; Carla Brazinha; Claudia F. Galinha; João G. Crespo

doi:10.1016/j.algal.2019.101720

Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

Marta Sá, Joana Monte, Carla Brazinha, Claudia F. Galinha, João G. Crespo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

Original language	English (US)
Pages (from-to)	101720
Journal	Algal Research
Volume	44
DOIs	https://doi.org/10.1016/j.algal.2019.101720
State	Published - Nov 12 2019
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2022-06-13
Acknowledged KAUST grant number(s): OSR-2016-CPF-2907-05
Acknowledgements: This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), by the European FP7 KBBE project “D-Factory” (contract no. 613870), by KAUST OSR award no. OSR-2016-CPF-2907-05, and by the follow Fellow grants of FCT/MCTES: SFRH/BPD/95864/2013, SFRH/BPD/79533/2011 and SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel). The authors would also like to thank the company A4F – Algae for future (Portugal), who performed all the pilot-scale cultivation trials and provided the biomass needed to develop this work.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

Agronomy and Crop Science

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10.1016/j.algal.2019.101720

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@article{bbf8891ac37441c69c32ecddf79ebd9c,

title = "Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina",

abstract = "Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.",

author = "Marta S{\'a} and Joana Monte and Carla Brazinha and Galinha, {Claudia F.} and Crespo, {Jo{\~a}o G.}",

note = "KAUST Repository Item: Exported on 2022-06-13 Acknowledged KAUST grant number(s): OSR-2016-CPF-2907-05 Acknowledgements: This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), by the European FP7 KBBE project “D-Factory” (contract no. 613870), by KAUST OSR award no. OSR-2016-CPF-2907-05, and by the follow Fellow grants of FCT/MCTES: SFRH/BPD/95864/2013, SFRH/BPD/79533/2011 and SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel). The authors would also like to thank the company A4F – Algae for future (Portugal), who performed all the pilot-scale cultivation trials and provided the biomass needed to develop this work. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2019",

month = nov,

day = "12",

doi = "10.1016/j.algal.2019.101720",

language = "English (US)",

volume = "44",

pages = "101720",

journal = "Algal Research",

issn = "2211-9264",

publisher = "Elsevier",

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T1 - Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

AU - Sá, Marta

AU - Monte, Joana

AU - Brazinha, Carla

AU - Galinha, Claudia F.

AU - Crespo, João G.

N1 - KAUST Repository Item: Exported on 2022-06-13 Acknowledged KAUST grant number(s): OSR-2016-CPF-2907-05 Acknowledgements: This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019), by the European FP7 KBBE project “D-Factory” (contract no. 613870), by KAUST OSR award no. OSR-2016-CPF-2907-05, and by the follow Fellow grants of FCT/MCTES: SFRH/BPD/95864/2013, SFRH/BPD/79533/2011 and SFRH/BD/108894/2015. The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel). The authors would also like to thank the company A4F – Algae for future (Portugal), who performed all the pilot-scale cultivation trials and provided the biomass needed to develop this work. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2019/11/12

Y1 - 2019/11/12

N2 - Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

AB - Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

UR - http://hdl.handle.net/10754/678921

UR - https://linkinghub.elsevier.com/retrieve/pii/S2211926419305934

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U2 - 10.1016/j.algal.2019.101720

DO - 10.1016/j.algal.2019.101720

M3 - Article

SN - 2211-9264

VL - 44

SP - 101720

JO - Algal Research

JF - Algal Research

ER -

Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

Abstract

Bibliographical note

ASJC Scopus subject areas

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