@inbook{185ae3b5052b4355a7f97320da2ed6c0,
title = "Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in arabidopsis and rice",
abstract = "Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.",
keywords = "Arabidopsis, Cell type-specific, FACS, GAL4, Protoplast, Rice, Salinity",
author = "Aurelie Evrard and Bargmann, {Bastiaan O.R.} and Birnbaum, {Kenneth D.} and Mark Tester and Ute Baumann and Johnson, {Alexander A.T.}",
year = "2012",
doi = "10.1007/978-1-61779-986-0_18",
language = "English (US)",
isbn = "9781617799853",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "265--276",
booktitle = "Plant Salt Tolerance",
}