Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Maxim Kogan; Tamás Beke-Somfai; Bengt Nordén

doi:10.1039/c1cc12313k

Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Maxim Kogan, Tamás Beke-Somfai, Bengt Nordén

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.

Original language	English (US)
Pages (from-to)	7356
Journal	Chemical Communications
Volume	47
Issue number	26
DOIs	https://doi.org/10.1039/c1cc12313k
State	Published - 2011
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): KUK-11-008-23
Acknowledgements: This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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title = "Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light",

abstract = "Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. {\textcopyright} 2011 The Royal Society of Chemistry.",

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AU - Beke-Somfai, Tamás

AU - Nordén, Bengt

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): KUK-11-008-23 Acknowledgements: This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO). This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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Y1 - 2011

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AB - Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.

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Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light

Abstract

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