Field approach to three-dimensional gene expression pattern characterization

L. Da F. Costa; B. A.N. Traveņolo; A. Azeredo; M. E. Beletti; G. B. Müller; D. Rasskin-Gutman; G. Sternik; M. Ibañes; J. C. Izpisúa-Belmonte

doi:10.1063/1.1898424

Field approach to three-dimensional gene expression pattern characterization

L. Da F. Costa^*, B. A.N. Traveņolo, A. Azeredo, M. E. Beletti, G. B. Müller, D. Rasskin-Gutman, G. Sternik, M. Ibañes, J. C. Izpisúa-Belmonte

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We present a vector field method for obtaining the spatial organization of three-dimensional patterns of gene expression based on gradients and lines of force obtained by numerical integration. The convergence of these lines of force in local maxima are centers of gene expression, providing a natural and powerful framework to characterize the organization and dynamics of biological structures. We apply this methodology to analyze the expression pattern of the enhanced green fluorescent protein (EGFP) driven by the promoter of light chain myosin II during zebrafish heart formation.

Original language	English (US)
Article number	143901
Pages (from-to)	1-3
Number of pages	3
Journal	Applied Physics Letters
Volume	86
Issue number	14
DOIs	https://doi.org/10.1063/1.1898424
State	Published - Apr 4 2005
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank HFSP RGP39∕2002 for funding this project. A. A. is grateful to FAPESP (02∕09149-2), andB. A. N. Travençolo is grateful to CAPES and FAPESP (03∕13072-8) for financial support. M. Ibañes is partially supported by the Fulbright Program and Generalitat of Catalunya. L. da F. Costa is grateful to FAPESP (Proc. 99∕12765-2) and CNPq (Proc. 301422∕92-3) for financial support.

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.1898424

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abstract = "We present a vector field method for obtaining the spatial organization of three-dimensional patterns of gene expression based on gradients and lines of force obtained by numerical integration. The convergence of these lines of force in local maxima are centers of gene expression, providing a natural and powerful framework to characterize the organization and dynamics of biological structures. We apply this methodology to analyze the expression pattern of the enhanced green fluorescent protein (EGFP) driven by the promoter of light chain myosin II during zebrafish heart formation.",

author = "Costa, {L. Da F.} and Traveņolo, {B. A.N.} and A. Azeredo and Beletti, {M. E.} and M{\"u}ller, {G. B.} and D. Rasskin-Gutman and G. Sternik and M. Iba{\~n}es and Izpis{\'u}a-Belmonte, {J. C.}",

note = "Funding Information: The authors thank HFSP RGP39∕2002 for funding this project. A. A. is grateful to FAPESP (02∕09149-2), andB. A. N. Traven{\c c}olo is grateful to CAPES and FAPESP (03∕13072-8) for financial support. M. Iba{\~n}es is partially supported by the Fulbright Program and Generalitat of Catalunya. L. da F. Costa is grateful to FAPESP (Proc. 99∕12765-2) and CNPq (Proc. 301422∕92-3) for financial support.",

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doi = "10.1063/1.1898424",

language = "English (US)",

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AU - Costa, L. Da F.

AU - Traveņolo, B. A.N.

AU - Azeredo, A.

AU - Beletti, M. E.

AU - Müller, G. B.

AU - Rasskin-Gutman, D.

AU - Sternik, G.

AU - Ibañes, M.

AU - Izpisúa-Belmonte, J. C.

N1 - Funding Information: The authors thank HFSP RGP39∕2002 for funding this project. A. A. is grateful to FAPESP (02∕09149-2), andB. A. N. Travençolo is grateful to CAPES and FAPESP (03∕13072-8) for financial support. M. Ibañes is partially supported by the Fulbright Program and Generalitat of Catalunya. L. da F. Costa is grateful to FAPESP (Proc. 99∕12765-2) and CNPq (Proc. 301422∕92-3) for financial support.

PY - 2005/4/4

Y1 - 2005/4/4

N2 - We present a vector field method for obtaining the spatial organization of three-dimensional patterns of gene expression based on gradients and lines of force obtained by numerical integration. The convergence of these lines of force in local maxima are centers of gene expression, providing a natural and powerful framework to characterize the organization and dynamics of biological structures. We apply this methodology to analyze the expression pattern of the enhanced green fluorescent protein (EGFP) driven by the promoter of light chain myosin II during zebrafish heart formation.

AB - We present a vector field method for obtaining the spatial organization of three-dimensional patterns of gene expression based on gradients and lines of force obtained by numerical integration. The convergence of these lines of force in local maxima are centers of gene expression, providing a natural and powerful framework to characterize the organization and dynamics of biological structures. We apply this methodology to analyze the expression pattern of the enhanced green fluorescent protein (EGFP) driven by the promoter of light chain myosin II during zebrafish heart formation.

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ER -

Field approach to three-dimensional gene expression pattern characterization

Abstract

Bibliographical note

ASJC Scopus subject areas

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