Field and in vitro evidence for in-situ bioremediation using compound-specific 13C/12C ratio monitoring

L. G. Stehmeier*, M. Mc D. Francis, T. R. Jack, E. Diegor, L. Winsor, T. A. Abrajano

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


This work describes the use of δ13C values of residual hydrocarbons as a method for demonstrating in-situ biodegradation. Microbial growth, hydrocarbon loss and increase in δ13C values were demonstrated in vitro using benzene and styrene as carbon substrates. Isotope evidence of biodegradation were subsequently sought in four field sites contaminated with a wide variety of hydrocarbons. Analysis of residual hydrocarbons in the field indicated that an overall increase in the δ13C generally accompanied loss of hydrocarbons, an observation consistent with in-situ biodegradation. The field samples were analyzed using vapor or soil extracts, and the increases in δ13C were observed using both types of samples. Vapor sampling is of practical interest because stable isotope ratio monitoring of soil vapor could dramatically reduce the number of wells required for monitoring of ongoing remediation efforts. Our preliminary studies of contaminated field sites allude to the potential of compound-specific isotopic monitoring techniques as a cost-effective measure of in-situ biodegradation.

Original languageEnglish (US)
Pages (from-to)821-833
Number of pages13
JournalOrganic Geochemistry
Issue number8 A
StatePublished - Aug 1999
Externally publishedYes


  • Aerobic microorganisms, Stable isotope fractionation, Dicyclopentadiene
  • Benzene, Styrene, Toluene, Residual hydrocarbon

ASJC Scopus subject areas

  • Geochemistry and Petrology


Dive into the research topics of 'Field and in vitro evidence for in-situ bioremediation using compound-specific 13C/12C ratio monitoring'. Together they form a unique fingerprint.

Cite this