FGFR-related gene nou-darake restricts brain tissues to the head region of planarians

Francesc Cebrià; Chiyoko Kobayashi; Yoshihiko Umesono; Masumi Nakazawa; Katsuhiko Mineta; Kazuho Ikeo; Takashi Gojobori; Mari Itoh; Masanori Taira; Alejandro Sánchez Alvarado; Kiyokazu Agata

doi:10.1038/nature01042

FGFR-related gene nou-darake restricts brain tissues to the head region of planarians

Francesc Cebrià, Chiyoko Kobayashi, Yoshihiko Umesono, Masumi Nakazawa, Katsuhiko Mineta, Kazuho Ikeo, Takashi Gojobori, Mari Itoh, Masanori Taira, Alejandro Sánchez Alvarado, Kiyokazu Agata

Research output: Contribution to journal › Article › peer-review

231 Scopus citations

Abstract

The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFRI and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.

Original language	English (US)
Pages (from-to)	620-624
Number of pages	5
Journal	NATURE
Volume	419
Issue number	6907
DOIs	https://doi.org/10.1038/nature01042
State	Published - Oct 10 2002
Externally published	Yes

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title = "FGFR-related gene nou-darake restricts brain tissues to the head region of planarians",

abstract = "The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFRI and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.",

author = "Francesc Cebri{\`a} and Chiyoko Kobayashi and Yoshihiko Umesono and Masumi Nakazawa and Katsuhiko Mineta and Kazuho Ikeo and Takashi Gojobori and Mari Itoh and Masanori Taira and {S{\'a}nchez Alvarado}, Alejandro and Kiyokazu Agata",

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AU - Cebrià, Francesc

AU - Kobayashi, Chiyoko

AU - Umesono, Yoshihiko

AU - Nakazawa, Masumi

AU - Mineta, Katsuhiko

AU - Ikeo, Kazuho

AU - Gojobori, Takashi

AU - Itoh, Mari

AU - Taira, Masanori

AU - Sánchez Alvarado, Alejandro

AU - Agata, Kiyokazu

PY - 2002/10/10

Y1 - 2002/10/10

N2 - The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFRI and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.

AB - The study of planarian regeneration may help us to understand how we can rebuild organs and tissues after injury, disease or ageing. The robust regenerative abilities of planarians are based upon a population of totipotent stem cells (neoblasts), and among the organs regenerated by these animals is a well-organized central nervous system. In recent years, methodologies such as whole-mount in situ hybridizations and double-stranded RNA have been extended to planarians with the aim of unravelling the molecular basis of their regenerative capacities. Here we report the identification and characterization of nou-darake (ndk), a gene encoding a fibroblast growth factor receptor (FGFR)-like molecule specifically expressed in the head region of the planarian Dugesia japonica. Loss of function of ndk by RNA interference results in the induction of ectopic brain tissues throughout the body. This ectopic brain formation was suppressed by inhibition of two planarian FGFR homologues (FGFRI and FGFR2). Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians.

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FGFR-related gene nou-darake restricts brain tissues to the head region of planarians

Abstract

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