Fast detection of genetic information by an optimized PCR in an interchangeable chip.

Jinbo Wu; Rimantas Kodzius; Jianhua Qin; Weijia Wen; Kang Xiao

doi:10.1007/s10544-011-9595-6

Fast detection of genetic information by an optimized PCR in an interchangeable chip.

Jinbo Wu, Rimantas Kodzius, Jianhua Qin, Weijia Wen, Kang Xiao

Physical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.

Original language	English (US)
Pages (from-to)	179-186
Number of pages	8
Journal	Biomedical Microdevices
Volume	14
Issue number	1
DOIs	https://doi.org/10.1007/s10544-011-9595-6
State	Published - Oct 6 2011

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040/UK-C0016
Acknowledgements: Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608

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https://repository.kaust.edu.sa/bitstream/10754/303136/7/Fast%20detection%20of%20genetic%20information%20by%20an%20optimized%20PCR%20in%20an%20interchangeable%20chip_revised.pdf

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title = "Fast detection of genetic information by an optimized PCR in an interchangeable chip.",

abstract = "In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.",

author = "Jinbo Wu and Rimantas Kodzius and Jianhua Qin and Weijia Wen and Kang Xiao",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040/UK-C0016 Acknowledgements: Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608",

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doi = "10.1007/s10544-011-9595-6",

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AU - Kodzius, Rimantas

AU - Qin, Jianhua

AU - Wen, Weijia

AU - Xiao, Kang

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040/UK-C0016 Acknowledgements: Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST); Hong Kong Research Grants Council Grant No. HKUST 603608

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N2 - In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.

AB - In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.

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Fast detection of genetic information by an optimized PCR in an interchangeable chip.

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