Fabrication of lumen-forming colorectal cancer organoids using a newly designed lamininderived bioink

Rosario Pérez-Pedroza, Fatimah Al-Jalih, Jiayi Xu, Manola Moretti, Giuseppina R. Briola, Charlotte A.E. Hauser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Three-dimensional (3D) bioprinting systems, which are the prominent tools for biofabrication, should evolve around the cutting-edge technologies of tissue engineering. This is the case with organoid technology, which requires a plethora of new materials to evolve, including extracellular matrices with specific mechanical and biochemical properties. For a bioprinting system to facilitate organoid growth, it must be able to recreate an organ-like environment within the 3D construct. In this study, a well-established, self-assembling peptide system was employed to generate a laminin-like bioink to provide signals of cell adhesion and lumen formation in cancer stem cells. One bioink formulation led to the formation of lumen with outperforming characteristics, which showed good stability of the printed construct.

Original languageEnglish (US)
Pages (from-to)160-172
Number of pages13
JournalInternational Journal of Bioprinting
Volume9
Issue number1
DOIs
StatePublished - 2023

Bibliographical note

Funding Information:
This work was supported by KAUST baseline funding and CBRC funding.

Publisher Copyright:
© 2022 Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, permitting distribution and reproduction in any medium, provided the original work is properly cited.

Keywords

  • Biofunctional bioink
  • Ikvav
  • Laminin
  • Organoid
  • Self-assembling peptide

ASJC Scopus subject areas

  • Biotechnology
  • Materials Science (miscellaneous)
  • Industrial and Manufacturing Engineering

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