Extraction yield and biological activity of phycobiliproteins from Porphyridium purpureum using atmospheric cold plasma discharge and jet systems

Shaba Noore; Brijesh K. Tiwari; Anet R. Jambrak; Josipa Dukić; Janith Wanigasekara; James F. Curtin; Colm O'Donnell

doi:10.1016/j.lwt.2023.115204

Extraction yield and biological activity of phycobiliproteins from Porphyridium purpureum using atmospheric cold plasma discharge and jet systems

Shaba Noore, Brijesh K. Tiwari, Anet R. Jambrak, Josipa Dukić, Janith Wanigasekara, James F. Curtin, Colm O'Donnell

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Phycobiliproteins (PBPs) extracted from Porphyridium purpureum (P.p) have bioactive properties and are widely used as ingredients in nutraceutical and food applications. This study investigated the use of two cold plasma systems, namely cold plasma discharge system (CPDS) and cold plasma jet system (CPJS), for the aqueous extraction of PBPs from P. p. Three types of PBPs, namely phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were identified in the crude extracts obtained. The highest PBPs extraction yield (11.31 ± 1.02 mg/g DW P. p) was obtained from CPDS treated samples at 25 kV using N2 for 9 min. CPDS treatments were also shown to be more effective than CPJS treatments in increasing antioxidant activities of the PBPs crude extracts obtained. PBPs crude extracts obtained using CPDS (25 kV; 6 min; N2) had the highest DPPH (69.44 ± 0.10%) and FRAP (207.34 ± 12.96 μmol/L) antioxidant activities observed. PBPs obtained from samples treated with CPDS (25 kV; 9 min; N2) exhibited the highest cytotoxicity potential in Caco-2 human colorectal adenocarcinoma cell lines. This study demonstrates that cold plasma treatments increase the extraction yield of PBPs obtained from P. p and also enhance antioxidant and cytotoxic properties of the PBP crude extracts. However, an increase in treatment time beyond 6 min for both plasma systems was shown to reduce the level of antioxidant activity in PBPs.

Original language	English (US)
Pages (from-to)	115204
Journal	LWT
Volume	187
DOIs	https://doi.org/10.1016/j.lwt.2023.115204
State	Published - Sep 16 2023
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2023-09-26
Acknowledgements: This research was financially supported by the BiOrbic SFI Bioeconomy Research Centre, which is funded by Ireland's European Structural and Investment Programmes, Science Foundation Ireland (16/RC/3889), and the European Development Fund. Microalgae cultures were funded by the Interreg Atlantic Area European Regional development fund, project Enhance Microalgae EAPA_338/2016.

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2023.115204

https://repository.kaust.edu.sa/bitstream/10754/694623/1/1-s2.0-S0023643823007831-main.pdf

Cite this

@article{67a661c8142a48fdbb116d91bff02626,

title = "Extraction yield and biological activity of phycobiliproteins from Porphyridium purpureum using atmospheric cold plasma discharge and jet systems",

abstract = "Phycobiliproteins (PBPs) extracted from Porphyridium purpureum (P.p) have bioactive properties and are widely used as ingredients in nutraceutical and food applications. This study investigated the use of two cold plasma systems, namely cold plasma discharge system (CPDS) and cold plasma jet system (CPJS), for the aqueous extraction of PBPs from P. p. Three types of PBPs, namely phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were identified in the crude extracts obtained. The highest PBPs extraction yield (11.31 ± 1.02 mg/g DW P. p) was obtained from CPDS treated samples at 25 kV using N2 for 9 min. CPDS treatments were also shown to be more effective than CPJS treatments in increasing antioxidant activities of the PBPs crude extracts obtained. PBPs crude extracts obtained using CPDS (25 kV; 6 min; N2) had the highest DPPH (69.44 ± 0.10%) and FRAP (207.34 ± 12.96 μmol/L) antioxidant activities observed. PBPs obtained from samples treated with CPDS (25 kV; 9 min; N2) exhibited the highest cytotoxicity potential in Caco-2 human colorectal adenocarcinoma cell lines. This study demonstrates that cold plasma treatments increase the extraction yield of PBPs obtained from P. p and also enhance antioxidant and cytotoxic properties of the PBP crude extracts. However, an increase in treatment time beyond 6 min for both plasma systems was shown to reduce the level of antioxidant activity in PBPs.",

author = "Shaba Noore and Tiwari, {Brijesh K.} and Jambrak, {Anet R.} and Josipa Duki{\'c} and Janith Wanigasekara and Curtin, {James F.} and Colm O'Donnell",

note = "KAUST Repository Item: Exported on 2023-09-26 Acknowledgements: This research was financially supported by the BiOrbic SFI Bioeconomy Research Centre, which is funded by Ireland's European Structural and Investment Programmes, Science Foundation Ireland (16/RC/3889), and the European Development Fund. Microalgae cultures were funded by the Interreg Atlantic Area European Regional development fund, project Enhance Microalgae EAPA_338/2016.",

year = "2023",

month = sep,

day = "16",

doi = "10.1016/j.lwt.2023.115204",

language = "English (US)",

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T1 - Extraction yield and biological activity of phycobiliproteins from Porphyridium purpureum using atmospheric cold plasma discharge and jet systems

AU - Noore, Shaba

AU - Tiwari, Brijesh K.

AU - Jambrak, Anet R.

AU - Dukić, Josipa

AU - Wanigasekara, Janith

AU - Curtin, James F.

AU - O'Donnell, Colm

N1 - KAUST Repository Item: Exported on 2023-09-26 Acknowledgements: This research was financially supported by the BiOrbic SFI Bioeconomy Research Centre, which is funded by Ireland's European Structural and Investment Programmes, Science Foundation Ireland (16/RC/3889), and the European Development Fund. Microalgae cultures were funded by the Interreg Atlantic Area European Regional development fund, project Enhance Microalgae EAPA_338/2016.

PY - 2023/9/16

Y1 - 2023/9/16

N2 - Phycobiliproteins (PBPs) extracted from Porphyridium purpureum (P.p) have bioactive properties and are widely used as ingredients in nutraceutical and food applications. This study investigated the use of two cold plasma systems, namely cold plasma discharge system (CPDS) and cold plasma jet system (CPJS), for the aqueous extraction of PBPs from P. p. Three types of PBPs, namely phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were identified in the crude extracts obtained. The highest PBPs extraction yield (11.31 ± 1.02 mg/g DW P. p) was obtained from CPDS treated samples at 25 kV using N2 for 9 min. CPDS treatments were also shown to be more effective than CPJS treatments in increasing antioxidant activities of the PBPs crude extracts obtained. PBPs crude extracts obtained using CPDS (25 kV; 6 min; N2) had the highest DPPH (69.44 ± 0.10%) and FRAP (207.34 ± 12.96 μmol/L) antioxidant activities observed. PBPs obtained from samples treated with CPDS (25 kV; 9 min; N2) exhibited the highest cytotoxicity potential in Caco-2 human colorectal adenocarcinoma cell lines. This study demonstrates that cold plasma treatments increase the extraction yield of PBPs obtained from P. p and also enhance antioxidant and cytotoxic properties of the PBP crude extracts. However, an increase in treatment time beyond 6 min for both plasma systems was shown to reduce the level of antioxidant activity in PBPs.

AB - Phycobiliproteins (PBPs) extracted from Porphyridium purpureum (P.p) have bioactive properties and are widely used as ingredients in nutraceutical and food applications. This study investigated the use of two cold plasma systems, namely cold plasma discharge system (CPDS) and cold plasma jet system (CPJS), for the aqueous extraction of PBPs from P. p. Three types of PBPs, namely phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were identified in the crude extracts obtained. The highest PBPs extraction yield (11.31 ± 1.02 mg/g DW P. p) was obtained from CPDS treated samples at 25 kV using N2 for 9 min. CPDS treatments were also shown to be more effective than CPJS treatments in increasing antioxidant activities of the PBPs crude extracts obtained. PBPs crude extracts obtained using CPDS (25 kV; 6 min; N2) had the highest DPPH (69.44 ± 0.10%) and FRAP (207.34 ± 12.96 μmol/L) antioxidant activities observed. PBPs obtained from samples treated with CPDS (25 kV; 9 min; N2) exhibited the highest cytotoxicity potential in Caco-2 human colorectal adenocarcinoma cell lines. This study demonstrates that cold plasma treatments increase the extraction yield of PBPs obtained from P. p and also enhance antioxidant and cytotoxic properties of the PBP crude extracts. However, an increase in treatment time beyond 6 min for both plasma systems was shown to reduce the level of antioxidant activity in PBPs.

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U2 - 10.1016/j.lwt.2023.115204

DO - 10.1016/j.lwt.2023.115204

M3 - Article

SN - 0023-6438

VL - 187

SP - 115204

JO - LWT

JF - LWT

ER -

Extraction yield and biological activity of phycobiliproteins from Porphyridium purpureum using atmospheric cold plasma discharge and jet systems

Abstract

Bibliographical note

ASJC Scopus subject areas

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