Extraction, amplification and detection of DNA in microfluidic chip-based assays

Jinbo Wu, Rimantas Kodzius, Wenbin Cao, Weijia Wen

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

This review covers three aspects of PCR-based microfluidic chip assays: sample preparation, target amplification, and product detection. We also discuss the challenges related to the miniaturization and integration of each assay and make a comparison between conventional and microfluidic schemes. In order to accomplish these essential assays without human intervention between individual steps, the micro-components for fluid manipulation become critical. We therefore summarize and discuss components such as microvalves (for fluid regulation), pumps (for fluid driving) and mixers (for blending fluids). By combining the above assays and microcomponents, DNA testing of multi-step bio-reactions in microfluidic chips may be achieved with minimal external control. The combination of assay schemes with the use of micro-components also leads to rapid methods for DNA testing via multi-step bioreactions. Contains 259 references.
Original languageEnglish (US)
Pages (from-to)1611-1631
Number of pages21
JournalMicrochimica Acta
Volume181
Issue number13-14
DOIs
StatePublished - Dec 20 2013

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040/UK-C0016
Acknowledgements: The authors acknowledge the financial support provided by the Hong Kong Research Grants Council Grant No. HKUST604710, 605411. This publication is based on work partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST).

ASJC Scopus subject areas

  • Analytical Chemistry

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