Extracellular Polymeric Substances Govern the Surface Charge of Biogenic Elemental Selenium Nanoparticles

Rohan Jain; Norbert Jordan; Stephan Weiss; Harald Foerstendorf; Karsten Heim; Rohit Kacker; René Hübner; Herman Kramer; Eric D. van Hullebusch; François Farges; Piet N. L. Lens

doi:10.1021/es5043063

Extracellular Polymeric Substances Govern the Surface Charge of Biogenic Elemental Selenium Nanoparticles

Rohan Jain, Norbert Jordan, Stephan Weiss, Harald Foerstendorf, Karsten Heim, Rohit Kacker, René Hübner, Herman Kramer, Eric D. van Hullebusch, François Farges, Piet N. L. Lens

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167 Scopus citations

Abstract

© 2014 American Chemical Society. The origin of the organic layer covering colloidal biogenic elemental selenium nanoparticles (BioSeNPs) is not known, particularly in the case when they are synthesized by complex microbial communities. This study investigated the presence of extracellular polymeric substances (EPS) on BioSeNPs. The role of EPS in capping the extracellularly available BioSeNPs was also examined. Fourier transform infrared (FT-IR) spectroscopy and colorimetric measurements confirmed the presence of functional groups characteristic of proteins and carbohydrates on the BioSeNPs, suggesting the presence of EPS. Chemical synthesis of elemental selenium nanoparticles in the presence of EPS, extracted from selenite fed anaerobic granular sludge, yielded stable colloidal spherical selenium nanoparticles. Furthermore, extracted EPS, BioSeNPs, and chemically synthesized EPS-capped selenium nanoparticles had similar surface properties, as shown by ζ-potential versus pH profiles and isoelectric point measurements. This study shows that the EPS of anaerobic granular sludge form the organic layer present on the BioSeNPs synthesized by these granules. The EPS also govern the surface charge of these BioSeNPs, thereby contributing to their colloidal properties, hence affecting their fate in the environment and the efficiency of bioremediation technologies.

Original language	English (US)
Pages (from-to)	1713-1720
Number of pages	8
Journal	Environmental Science & Technology
Volume	49
Issue number	3
DOIs	https://doi.org/10.1021/es5043063
State	Published - Jan 21 2015
Externally published	Yes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors thank Dr. Graciella Gil-Gonzalez (KAUST, Saudi Arabia) for insightful discussion, Ferdi Battles (UNESCO-IHE, The Netherlands) for the Nanosizer and acid–base titration experiments, Berend Lolkema (UNESCO-IHE, The Netherlands) for TOC, TN, and 3D EEM analysis, Elfi Christalle (Helmholtz-Zentrum, Dresden-Rossendorf, Germany) for SEM-EDXS measurements, and Purvi Jain (Utrecht University, The Netherlands) for DNA measurements.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1021/es5043063

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@article{f05178c7a59b4d2f899c616bd8476ee5,

title = "Extracellular Polymeric Substances Govern the Surface Charge of Biogenic Elemental Selenium Nanoparticles",

abstract = "{\textcopyright} 2014 American Chemical Society. The origin of the organic layer covering colloidal biogenic elemental selenium nanoparticles (BioSeNPs) is not known, particularly in the case when they are synthesized by complex microbial communities. This study investigated the presence of extracellular polymeric substances (EPS) on BioSeNPs. The role of EPS in capping the extracellularly available BioSeNPs was also examined. Fourier transform infrared (FT-IR) spectroscopy and colorimetric measurements confirmed the presence of functional groups characteristic of proteins and carbohydrates on the BioSeNPs, suggesting the presence of EPS. Chemical synthesis of elemental selenium nanoparticles in the presence of EPS, extracted from selenite fed anaerobic granular sludge, yielded stable colloidal spherical selenium nanoparticles. Furthermore, extracted EPS, BioSeNPs, and chemically synthesized EPS-capped selenium nanoparticles had similar surface properties, as shown by ζ-potential versus pH profiles and isoelectric point measurements. This study shows that the EPS of anaerobic granular sludge form the organic layer present on the BioSeNPs synthesized by these granules. The EPS also govern the surface charge of these BioSeNPs, thereby contributing to their colloidal properties, hence affecting their fate in the environment and the efficiency of bioremediation technologies.",

author = "Rohan Jain and Norbert Jordan and Stephan Weiss and Harald Foerstendorf and Karsten Heim and Rohit Kacker and Ren{\'e} H{\"u}bner and Herman Kramer and {van Hullebusch}, {Eric D.} and Fran{\c c}ois Farges and Lens, {Piet N. L.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors thank Dr. Graciella Gil-Gonzalez (KAUST, Saudi Arabia) for insightful discussion, Ferdi Battles (UNESCO-IHE, The Netherlands) for the Nanosizer and acid–base titration experiments, Berend Lolkema (UNESCO-IHE, The Netherlands) for TOC, TN, and 3D EEM analysis, Elfi Christalle (Helmholtz-Zentrum, Dresden-Rossendorf, Germany) for SEM-EDXS measurements, and Purvi Jain (Utrecht University, The Netherlands) for DNA measurements. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2015",

month = jan,

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doi = "10.1021/es5043063",

language = "English (US)",

volume = "49",

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T1 - Extracellular Polymeric Substances Govern the Surface Charge of Biogenic Elemental Selenium Nanoparticles

AU - Jain, Rohan

AU - Jordan, Norbert

AU - Weiss, Stephan

AU - Foerstendorf, Harald

AU - Heim, Karsten

AU - Kacker, Rohit

AU - Hübner, René

AU - Kramer, Herman

AU - van Hullebusch, Eric D.

AU - Farges, François

AU - Lens, Piet N. L.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The authors thank Dr. Graciella Gil-Gonzalez (KAUST, Saudi Arabia) for insightful discussion, Ferdi Battles (UNESCO-IHE, The Netherlands) for the Nanosizer and acid–base titration experiments, Berend Lolkema (UNESCO-IHE, The Netherlands) for TOC, TN, and 3D EEM analysis, Elfi Christalle (Helmholtz-Zentrum, Dresden-Rossendorf, Germany) for SEM-EDXS measurements, and Purvi Jain (Utrecht University, The Netherlands) for DNA measurements. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2015/1/21

Y1 - 2015/1/21

N2 - © 2014 American Chemical Society. The origin of the organic layer covering colloidal biogenic elemental selenium nanoparticles (BioSeNPs) is not known, particularly in the case when they are synthesized by complex microbial communities. This study investigated the presence of extracellular polymeric substances (EPS) on BioSeNPs. The role of EPS in capping the extracellularly available BioSeNPs was also examined. Fourier transform infrared (FT-IR) spectroscopy and colorimetric measurements confirmed the presence of functional groups characteristic of proteins and carbohydrates on the BioSeNPs, suggesting the presence of EPS. Chemical synthesis of elemental selenium nanoparticles in the presence of EPS, extracted from selenite fed anaerobic granular sludge, yielded stable colloidal spherical selenium nanoparticles. Furthermore, extracted EPS, BioSeNPs, and chemically synthesized EPS-capped selenium nanoparticles had similar surface properties, as shown by ζ-potential versus pH profiles and isoelectric point measurements. This study shows that the EPS of anaerobic granular sludge form the organic layer present on the BioSeNPs synthesized by these granules. The EPS also govern the surface charge of these BioSeNPs, thereby contributing to their colloidal properties, hence affecting their fate in the environment and the efficiency of bioremediation technologies.

AB - © 2014 American Chemical Society. The origin of the organic layer covering colloidal biogenic elemental selenium nanoparticles (BioSeNPs) is not known, particularly in the case when they are synthesized by complex microbial communities. This study investigated the presence of extracellular polymeric substances (EPS) on BioSeNPs. The role of EPS in capping the extracellularly available BioSeNPs was also examined. Fourier transform infrared (FT-IR) spectroscopy and colorimetric measurements confirmed the presence of functional groups characteristic of proteins and carbohydrates on the BioSeNPs, suggesting the presence of EPS. Chemical synthesis of elemental selenium nanoparticles in the presence of EPS, extracted from selenite fed anaerobic granular sludge, yielded stable colloidal spherical selenium nanoparticles. Furthermore, extracted EPS, BioSeNPs, and chemically synthesized EPS-capped selenium nanoparticles had similar surface properties, as shown by ζ-potential versus pH profiles and isoelectric point measurements. This study shows that the EPS of anaerobic granular sludge form the organic layer present on the BioSeNPs synthesized by these granules. The EPS also govern the surface charge of these BioSeNPs, thereby contributing to their colloidal properties, hence affecting their fate in the environment and the efficiency of bioremediation technologies.

UR - http://hdl.handle.net/10754/598300

UR - https://pubs.acs.org/doi/10.1021/es5043063

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U2 - 10.1021/es5043063

DO - 10.1021/es5043063

M3 - Article

C2 - 25536371

SN - 0013-936X

VL - 49

SP - 1713

EP - 1720

JO - Environmental Science & Technology

JF - Environmental Science & Technology

IS - 3

ER -

Extracellular Polymeric Substances Govern the Surface Charge of Biogenic Elemental Selenium Nanoparticles

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