Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths

Anna Nordborg; Frantisek Svec; Jean M.J. Fréchet; Knut Irgum

doi:10.1002/jssc.200500222

Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths

Anna Nordborg, Frantisek Svec^*, Jean M.J. Fréchet, Knut Irgum

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 μm ID capillaries and their application for μ-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 μm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.

Original language	English (US)
Pages (from-to)	2401-2406
Number of pages	6
Journal	Journal of Separation Science
Volume	28
Issue number	17
DOIs	https://doi.org/10.1002/jssc.200500222
State	Published - Nov 2005
Externally published	Yes

Keywords

Capillary
Crosslinker
Monolith
Monolithic column
Polymerization
Pore size
Protein separation
Reverse phase
μ-HPLC

ASJC Scopus subject areas

Analytical Chemistry
Filtration and Separation

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10.1002/jssc.200500222

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title = "Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths",

abstract = "The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 μm ID capillaries and their application for μ-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 μm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.",

keywords = "Capillary, Crosslinker, Monolith, Monolithic column, Polymerization, Pore size, Protein separation, Reverse phase, μ-HPLC",

author = "Anna Nordborg and Frantisek Svec and Fr{\'e}chet, {Jean M.J.} and Knut Irgum",

year = "2005",

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AU - Nordborg, Anna

AU - Svec, Frantisek

AU - Fréchet, Jean M.J.

AU - Irgum, Knut

PY - 2005/11

Y1 - 2005/11

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AB - The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 μm ID capillaries and their application for μ-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 μm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.

KW - Capillary

KW - Crosslinker

KW - Monolith

KW - Monolithic column

KW - Polymerization

KW - Pore size

KW - Protein separation

KW - Reverse phase

KW - μ-HPLC

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DO - 10.1002/jssc.200500222

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SN - 1615-9306

VL - 28

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JO - Journal of Separation Science

JF - Journal of Separation Science

IS - 17

ER -

Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths

Abstract

Keywords

ASJC Scopus subject areas

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