Abstract
Injured skeletal muscles which lose more than 20% of their volume, known as volumetric muscle loss, can no longer regenerate cells through self-healing. The traditional solution for recovery is through regenerative therapy. As the technology of three-dimensional (3D) bioprinting continues to advance, a new approach for tissue transplantation is using biocompatible materials arranged in 3D scaffolds for muscle repair. Ultrashort self-assembling peptide hydrogels compete as a potential biomaterial for muscle tissue formation due to their biocompatibility. In this study, two sequences of ultrashort peptides were analyzed with muscle myoblast cells (C2C12) for cell viability, cell proliferation, and differentiation in 3D cell culture. The peptides were then extruded through a custom-designed robotic 3D bioprinter to create cell-laden 3D structures. These constructs were also analyzed for cell viability through live/dead assay. Results showed that 3D bioprinted structures of peptide hydrogels could be used as tissue platforms for myotube formation – a process necessary for muscle repair.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 74-82 |
| Number of pages | 9 |
| Journal | International Journal of Bioprinting |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 26 2019 |
Bibliographical note
KAUST Repository Item: Exported on 2020-10-01Acknowledgements: The research reported in this publication was supported by funding from King Abdullah University for Science and Technology. We would like to thank Dr. Dana Alhattab and Francesca Melle for valuably contributing to the optimization of the 3D bioprinting process.