Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity

Pierre Marquet; Pascal Jourdain; Erik Belanger; Pierre Magistretti

doi:10.1109/WIO.2018.8643551

Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity

Pierre Marquet, Pascal Jourdain, Erik Belanger, Pierre Magistretti

Biological, Environmental Sciences and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Among the different techniques in the growing field of quantitative phase imaging (QPI), Quantitative Phase Digital Holographic Microscopy (QP-DHM) is particularly well suited to explore, with a nanometric axial sensitivity, cell structure and dynamics, by providing quantitative phase signal (QPS). QPS depends on both the thickness and the intracellular refractive index of the observed cells and brings thus information about both cell morphology and cell contents. Thanks to the development of different experimental procedures, relevant biophysical cell parameters can be successfully calculated from QPS, including cell shape, absolute volume, intracellular protein concentration, organelle distribution, nanoscale membrane fluctuations, membrane mechanical properties and water permeability, as well as transmembrane water movements. Simultaneous dynamic imaging of transmembrane water movements and cell volume is likely to assess the cell capacity to maintain or not homeostasis and consequently to identify early biomarkers of cell viability and cytotoxicity.

Original language	English (US)
Title of host publication	2018 17th Workshop on Information Optics, WIO 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781538660133
DOIs	https://doi.org/10.1109/WIO.2018.8643551
State	Published - Feb 15 2019
Event	17th Workshop on Information Optics, WIO 2018 - Quebec, Canada Duration: Jul 16 2018 → Jul 19 2018

Publication series

Name	2018 17th Workshop on Information Optics, WIO 2018

Conference

Conference	17th Workshop on Information Optics, WIO 2018
Country/Territory	Canada
City	Quebec
Period	07/16/18 → 07/19/18

Bibliographical note

Funding Information:
This research has been funded by the Canada Excellence Research Chair in Neurophotonics, Université Laval, Université de Lausanne, Lausanne University Hospital and the Fondation de Préfargier.

Publisher Copyright:
© 2018 IEEE.

Keywords

cell biomarkers
cell imaging
digital holographic microscopy
glutamate
quantitative phase imaging

ASJC Scopus subject areas

Information Systems
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

Access to Document

10.1109/WIO.2018.8643551

Cite this

Marquet, P., Jourdain, P., Belanger, E., & Magistretti, P. (2019). Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity. In 2018 17th Workshop on Information Optics, WIO 2018 Article 8643551 (2018 17th Workshop on Information Optics, WIO 2018). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/WIO.2018.8643551

Marquet, Pierre ; Jourdain, Pascal ; Belanger, Erik et al. / Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy : Towards identifying early biomarkers of cell viability and cytotoxicity. 2018 17th Workshop on Information Optics, WIO 2018. Institute of Electrical and Electronics Engineers Inc., 2019. (2018 17th Workshop on Information Optics, WIO 2018).

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abstract = "Among the different techniques in the growing field of quantitative phase imaging (QPI), Quantitative Phase Digital Holographic Microscopy (QP-DHM) is particularly well suited to explore, with a nanometric axial sensitivity, cell structure and dynamics, by providing quantitative phase signal (QPS). QPS depends on both the thickness and the intracellular refractive index of the observed cells and brings thus information about both cell morphology and cell contents. Thanks to the development of different experimental procedures, relevant biophysical cell parameters can be successfully calculated from QPS, including cell shape, absolute volume, intracellular protein concentration, organelle distribution, nanoscale membrane fluctuations, membrane mechanical properties and water permeability, as well as transmembrane water movements. Simultaneous dynamic imaging of transmembrane water movements and cell volume is likely to assess the cell capacity to maintain or not homeostasis and consequently to identify early biomarkers of cell viability and cytotoxicity.",

keywords = "cell biomarkers, cell imaging, digital holographic microscopy, glutamate, quantitative phase imaging",

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Marquet, P, Jourdain, P, Belanger, E & Magistretti, P 2019, Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity. in 2018 17th Workshop on Information Optics, WIO 2018., 8643551, 2018 17th Workshop on Information Optics, WIO 2018, Institute of Electrical and Electronics Engineers Inc., 17th Workshop on Information Optics, WIO 2018, Quebec, Canada, 07/16/18. https://doi.org/10.1109/WIO.2018.8643551

Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity. / Marquet, Pierre; Jourdain, Pascal; Belanger, Erik et al.
2018 17th Workshop on Information Optics, WIO 2018. Institute of Electrical and Electronics Engineers Inc., 2019. 8643551 (2018 17th Workshop on Information Optics, WIO 2018).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N1 - Funding Information: This research has been funded by the Canada Excellence Research Chair in Neurophotonics, Université Laval, Université de Lausanne, Lausanne University Hospital and the Fondation de Préfargier. Publisher Copyright: © 2018 IEEE.

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Marquet P, Jourdain P, Belanger E, Magistretti P. Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity. In 2018 17th Workshop on Information Optics, WIO 2018. Institute of Electrical and Electronics Engineers Inc. 2019. 8643551. (2018 17th Workshop on Information Optics, WIO 2018). doi: 10.1109/WIO.2018.8643551

Exploring cell structure, dynamics and homeostasis with a multimodal microscopy approach based on digital holographic microscopy: Towards identifying early biomarkers of cell viability and cytotoxicity

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this