Expanded bed adsorption/desorption of proteins with streamline direct CST I adsorbent

Ping Li, Guohua Xiu, Vera G. Mata, Carlos A. Grande, Alirio E. Rodrigues

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two-component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single- and two-component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two-component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I. © 2006 Wiley Periodicals, Inc.
Original languageEnglish (US)
Pages (from-to)1155-1163
Number of pages9
JournalBiotechnology and Bioengineering
Volume94
Issue number6
DOIs
StatePublished - Aug 20 2006
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2022-09-13

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

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