Evidence for species-dependent biosynthetic pathways for converting carlactone to strigolactones in plants

Moe Iseki, Kasumi Shida, Kazuma Kuwabara, Takatoshi Wakabayashi, Masaharu Mizutani, Hirosato Takikawa, Yukihiro Sugimoto

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32 Scopus citations


Strigolactones (SLs), comprising compounds with diverse but related chemical structures, are determinant signals in elicitation of germination in root parasitic Orobanchaceae and in mycorrhization in plants. Further, SLs are a novel class of plant hormones that regulate root and shoot architecture. Dissecting common and divergent biosynthetic pathways of SLs may provide avenues for modulating their production in planta. Biosynthesis of SLs in various SL-producing plant species was inhibited by fluridone, a phytoene desaturase inhibitor. The plausible biosynthetic precursors of SLs were exogenously applied to plants, and their conversion to canonical and non-canonical SLs was analysed using liquid chromatography-tandem mass spectrometry. The conversion of carlactone (CL) to carlactonoic acid (CLA) was a common reaction in all investigated plants. Sorghum converted CLA to 5-deoxystrigol (5-DS) and sorgomol, and 5-DS to sorgomol. One sorgomol-producing cotton cultivar had the same SL profile as sorghum in the feeding experiments. Another cotton cultivar converted CLA to 5-DS, strigol, and strigyl acetate. Further, 5-DS was converted to strigol and strigyl acetate. Moonseed converted CLA to strigol, but not to 5-DS. The plant did not convert 5-DS to strigol, suggesting that 5-DS is not a precursor of strigol in moonseed. Similarly, 4-deoxyorobanchol was not a precursor of orobanchol in cowpea. Further, sunflower converted CLA to methyl carlactonoate and heliolactone. These results indicated that the biosynthetic pathways of hydroxy SLs do not necessarily involve their respective deoxy SL precursors.
Original languageEnglish (US)
Pages (from-to)2305-2318
Number of pages14
JournalJournal of Experimental Botany
Issue number9
StatePublished - Dec 23 2017
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2022-06-07
Acknowledgements: This work was supported, in part, by grants from JICA/JST, the Science and Technology Research Partnership for Sustainable Development (SATREPS), and Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (No. 25292065). The authors are grateful to Prof. Salim Al-Babili, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia, for providing an authentic sample of CL. We also thank Prof. Abdel Gabar Babiker, National Center for Research, Sudan, for valuable discussions.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

  • Plant Science
  • Physiology


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