Evidence for in vivo interactions between dehydrins and the aquaporin AtPIP2B

Itzell Eurídice Hernández-Sánchez; Israel Maruri-López; Eugenio Pérez Molphe-Balch; Alicia Becerra-Flora; Fabiola Jaimes-Miranda; Juan F. Jiménez-Bremont

doi:10.1016/j.bbrc.2019.01.095

Evidence for in vivo interactions between dehydrins and the aquaporin AtPIP2B

Itzell Eurídice Hernández-Sánchez, Israel Maruri-López, Eugenio Pérez Molphe-Balch, Alicia Becerra-Flora, Fabiola Jaimes-Miranda^*, Juan F. Jiménez-Bremont

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Plants have developed mechanisms that allow them to tolerate different abiotic stresses. Among these mechanisms, the accumulation of specific proteins such as dehydrins (DHNs) and aquaporins (AQPs) can protect other proteins from damage during dehydration and may allow the control of water loss, respectively. Although both types of proteins are involved in plant protection against dehydration stress, a direct interaction between them has not been explored. A previous screen to identify potential OpsDHN1 protein interactions revealed an aquaporin as a possible candidate. Here, we used the Bimolecular Fluorescence Complementation (BiFC) approach to investigate the direct interaction of the cactus OpsDHN1 protein with the Arabidopsis plasma membrane PIP family aquaporin AtPIP2B (At2G37170). Since AtPIP2B is a membrane protein and OpsDHN1 is a cytosolic protein that may be peripherally associated with membranes, we propose that OpsDHN1/AtPIP2B interaction takes place at cellular membranes. Furthermore, we also demonstrate the interaction of AtPIP2B with the three Arabidopsis dehydrins COR47 (AT1G20440), ERD10 (At1g20450), and RAB18 (At5g66400).

Original language	English (US)
Pages (from-to)	545-550
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	510
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2019.01.095
State	Published - Mar 19 2019

Bibliographical note

Funding Information:
This work was supported by the CONACYT funding (Proyectos de Desarrollo Científico para Atender Problemas Nacionales 2015-01-414). We thank Biologist Adriana Cecilia Moreno Flores for her technical assistance and to Dr. Alma Lilian Guerrero Barrera to provide confocal laser microscope services at Universidad Autónoma de Aguascalientes, México (Laboratory of cellular and tissue biology, building 203). We also thank Dr. Dirk K. Hincha (Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany) for his critical reading of the manuscript.

Publisher Copyright:
© 2019 Elsevier Inc.

Keywords

Aquaporin
BiFC
Dehydrin
Plasma membrane

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2019.01.095

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title = "Evidence for in vivo interactions between dehydrins and the aquaporin AtPIP2B",

abstract = "Plants have developed mechanisms that allow them to tolerate different abiotic stresses. Among these mechanisms, the accumulation of specific proteins such as dehydrins (DHNs) and aquaporins (AQPs) can protect other proteins from damage during dehydration and may allow the control of water loss, respectively. Although both types of proteins are involved in plant protection against dehydration stress, a direct interaction between them has not been explored. A previous screen to identify potential OpsDHN1 protein interactions revealed an aquaporin as a possible candidate. Here, we used the Bimolecular Fluorescence Complementation (BiFC) approach to investigate the direct interaction of the cactus OpsDHN1 protein with the Arabidopsis plasma membrane PIP family aquaporin AtPIP2B (At2G37170). Since AtPIP2B is a membrane protein and OpsDHN1 is a cytosolic protein that may be peripherally associated with membranes, we propose that OpsDHN1/AtPIP2B interaction takes place at cellular membranes. Furthermore, we also demonstrate the interaction of AtPIP2B with the three Arabidopsis dehydrins COR47 (AT1G20440), ERD10 (At1g20450), and RAB18 (At5g66400).",

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author = "Hern{\'a}ndez-S{\'a}nchez, {Itzell Eur{\'i}dice} and Israel Maruri-L{\'o}pez and Molphe-Balch, {Eugenio P{\'e}rez} and Alicia Becerra-Flora and Fabiola Jaimes-Miranda and Jim{\'e}nez-Bremont, {Juan F.}",

note = "Funding Information: This work was supported by the CONACYT funding (Proyectos de Desarrollo Cient{\'i}fico para Atender Problemas Nacionales 2015-01-414). We thank Biologist Adriana Cecilia Moreno Flores for her technical assistance and to Dr. Alma Lilian Guerrero Barrera to provide confocal laser microscope services at Universidad Aut{\'o}noma de Aguascalientes, M{\'e}xico (Laboratory of cellular and tissue biology, building 203). We also thank Dr. Dirk K. Hincha (Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany) for his critical reading of the manuscript. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

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AU - Hernández-Sánchez, Itzell Eurídice

AU - Maruri-López, Israel

AU - Molphe-Balch, Eugenio Pérez

AU - Becerra-Flora, Alicia

AU - Jaimes-Miranda, Fabiola

AU - Jiménez-Bremont, Juan F.

N1 - Funding Information: This work was supported by the CONACYT funding (Proyectos de Desarrollo Científico para Atender Problemas Nacionales 2015-01-414). We thank Biologist Adriana Cecilia Moreno Flores for her technical assistance and to Dr. Alma Lilian Guerrero Barrera to provide confocal laser microscope services at Universidad Autónoma de Aguascalientes, México (Laboratory of cellular and tissue biology, building 203). We also thank Dr. Dirk K. Hincha (Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany) for his critical reading of the manuscript. Publisher Copyright: © 2019 Elsevier Inc.

PY - 2019/3/19

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N2 - Plants have developed mechanisms that allow them to tolerate different abiotic stresses. Among these mechanisms, the accumulation of specific proteins such as dehydrins (DHNs) and aquaporins (AQPs) can protect other proteins from damage during dehydration and may allow the control of water loss, respectively. Although both types of proteins are involved in plant protection against dehydration stress, a direct interaction between them has not been explored. A previous screen to identify potential OpsDHN1 protein interactions revealed an aquaporin as a possible candidate. Here, we used the Bimolecular Fluorescence Complementation (BiFC) approach to investigate the direct interaction of the cactus OpsDHN1 protein with the Arabidopsis plasma membrane PIP family aquaporin AtPIP2B (At2G37170). Since AtPIP2B is a membrane protein and OpsDHN1 is a cytosolic protein that may be peripherally associated with membranes, we propose that OpsDHN1/AtPIP2B interaction takes place at cellular membranes. Furthermore, we also demonstrate the interaction of AtPIP2B with the three Arabidopsis dehydrins COR47 (AT1G20440), ERD10 (At1g20450), and RAB18 (At5g66400).

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ER -

Evidence for in vivo interactions between dehydrins and the aquaporin AtPIP2B

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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