Evaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy

Gaik Chin Yap, Kok Keong Chee, Pei Ying Hong, Christophe Lay, Cahya D. Satria, Sumadiono, Yati Soenarto, Ekawaty L. Haksari, Marion Aw, Lynette Pei Chi Shek, Kaw Yan Chua, Yudong Zhao, Doreen Leow, Bee Wah Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Background: Studies have suggested that demographic and lifestyle factors could shape the composition of fecal microbiota in early life. This study evaluated infant stool microbiota signatures in two Asian populations, Singapore (n = 42) and Indonesia (n = 32) with contrasting socioeconomic development, and examined the putative influences of demographic factors on these human fecal associated bacterial signatures. Results: Longitudinal analysis showed associations of geographical origin with Clostridium leptum, Atopobium and Bifidobacterium groups. Mode of delivery had the largest effect on stool microbiota signatures influencing the abundance of four bacterial groups. Significantly higher abundance of bacterial members belonging to the Bacteroides-Prevotella, Bifidobacterium and Atopobium groups, but lower abundance of Lactobacilli-Enterococci group members, were observed in vaginal delivered compared to caesarean delivered infants. Demographic factors influencing the structure of infants stool microbiota during the first year of life included breastfeeding, age of weaning, sibship size and exposure to antibiotics. Conclusions: Differences in stool microbiota signatures were observed in relation to various demographic factors. These features may confound studies relating to the association of the structure of fecal microbiota and the predisposition to human modern disease.

Original languageEnglish (US)
Article number193
JournalBMC microbiology
StatePublished - 2011
Externally publishedYes

Bibliographical note

Funding Information:
The authors would like to thank Dr Shen Liang for statistical advice, Drs Dawn Lim and Irvin Gerez, Ms Hor Chuen Yee, Corinne Kwek Poh Lian and Judy Anthony who assisted with follow-up of the subjects. The voluntary participation of all subjects in this study is sincerely appreciated. This study was supported by A*STAR’s Biomedical Research Council (BMRC) and the MOH’s National Medical Research Council (BMRC/08/1/21/19/566).

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)


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