Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Noriko Karasaki; Hiroaki Mon; Masateru Takahashi; Jae Man Lee; Katsumi Koga; Yutaka Kawaguchi; Takahiro Kusakabe

doi:10.1007/s10529-008-9898-8

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Noriko Karasaki, Hiroaki Mon, Masateru Takahashi, Jae Man Lee, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe^*

^*Corresponding author for this work

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9 Scopus citations

Abstract

Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.

Original language	English (US)
Pages (from-to)	495-500
Number of pages	6
Journal	Biotechnology Letters
Volume	31
Issue number	4
DOIs	https://doi.org/10.1007/s10529-008-9898-8
State	Published - Apr 2009

Keywords

Bombyx mori
Doxycycline
RtTA
Tet-on system
TetO

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Bioengineering
Biotechnology

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10.1007/s10529-008-9898-8

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title = "Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori",

abstract = "Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.",

keywords = "Bombyx mori, Doxycycline, RtTA, Tet-on system, TetO",

author = "Noriko Karasaki and Hiroaki Mon and Masateru Takahashi and Lee, {Jae Man} and Katsumi Koga and Yutaka Kawaguchi and Takahiro Kusakabe",

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AU - Karasaki, Noriko

AU - Mon, Hiroaki

AU - Takahashi, Masateru

AU - Lee, Jae Man

AU - Koga, Katsumi

AU - Kawaguchi, Yutaka

AU - Kusakabe, Takahiro

PY - 2009/4

Y1 - 2009/4

N2 - Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.

AB - Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.

KW - Bombyx mori

KW - Doxycycline

KW - RtTA

KW - Tet-on system

KW - TetO

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Abstract

Keywords

ASJC Scopus subject areas

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