Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

Tomoyuki Miura; Jun Ichi Sakuragi; Meiko Kawamura; Masashi Fukasawa; Etsuko N. Moriyama; Takashi Gojobori; Koh Ichi Ishikawat; Julius A.A. Mingle; Victor B.A. Nettey; Hirofumi Akari; Masayoshi Enami; Hajime Tsujimoto; Masanori Hayami

Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

Tomoyuki Miura, Jun Ichi Sakuragi, Meiko Kawamura, Masashi Fukasawa, Etsuko N. Moriyama, Takashi Gojobori, Koh Ichi Ishikawat, Julius A.A. Mingle, Victor B.A. Nettey, Hirofumi Akari, Masayoshi Enami, Hajime Tsujimoto, Masanori Hayami^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2_ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIV_mac/SIV_sm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

Original language	English (US)
Pages (from-to)	1257-1261
Number of pages	5
Journal	AIDS
Volume	4
Issue number	12
State	Published - Dec 1990
Externally published	Yes

Keywords

AIDS
HIV
Lentivirus
Phylogenetic tree
Polymerase chain reaction
Simian immunodeficiency virus

ASJC Scopus subject areas

Infectious Diseases
Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup",

abstract = "We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIVmac/SIVsm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.",

keywords = "AIDS, HIV, Lentivirus, Phylogenetic tree, Polymerase chain reaction, Simian immunodeficiency virus",

author = "Tomoyuki Miura and Sakuragi, {Jun Ichi} and Meiko Kawamura and Masashi Fukasawa and Moriyama, {Etsuko N.} and Takashi Gojobori and Ishikawat, {Koh Ichi} and Mingle, {Julius A.A.} and Nettey, {Victor B.A.} and Hirofumi Akari and Masayoshi Enami and Hajime Tsujimoto and Masanori Hayami",

year = "1990",

month = dec,

language = "English (US)",

volume = "4",

pages = "1257--1261",

journal = "AIDS",

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publisher = "Lippincott Williams and Wilkins",

number = "12",

}

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T1 - Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

AU - Miura, Tomoyuki

AU - Sakuragi, Jun Ichi

AU - Kawamura, Meiko

AU - Fukasawa, Masashi

AU - Moriyama, Etsuko N.

AU - Gojobori, Takashi

AU - Ishikawat, Koh Ichi

AU - Mingle, Julius A.A.

AU - Nettey, Victor B.A.

AU - Akari, Hirofumi

AU - Enami, Masayoshi

AU - Tsujimoto, Hajime

AU - Hayami, Masanori

PY - 1990/12

Y1 - 1990/12

N2 - We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIVmac/SIVsm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

AB - We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIVmac/SIVsm in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.

KW - AIDS

KW - HIV

KW - Lentivirus

KW - Phylogenetic tree

KW - Polymerase chain reaction

KW - Simian immunodeficiency virus

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M3 - Article

C2 - 1965125

AN - SCOPUS:0025647022

SN - 0269-9370

VL - 4

SP - 1257

EP - 1261

JO - AIDS

JF - AIDS

IS - 12

ER -

Establishment of a phylogenetic survey system for AIDS-related lentiviruses and demonstration of a new HIV-2 subgroup

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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