EST-based identification of genes expressed in skeletal muscle of the mandarin fish (Siniperca chuatsi)

Feng Ding, Wuying Chu, Peng Cui, Meng Tao, Ruixue Zhou, Falan Zhao, Songnian Hu*, Jianshe Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags (ESTs) from the muscle cDNA database of the mandarin fish (Siniperca chuatsi). Clustering analysis yielded 1,625 unique sequences including 443 contigs (from 3,881 EST sequences) and 1,182 singletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 EST sequences accounting for 8.86% of the total EST sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.

Original languageEnglish (US)
Pages (from-to)30-36
Number of pages7
JournalGenomics, Proteomics and Bioinformatics
Issue number1-2
StatePublished - Apr 2011

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 30972263 and 30771644 ), and the Natural Science Foundation of Hunan Province, China (Grant No. 09JJ6037 and 08jj3064 ).


  • CDNA library
  • EST
  • Mandarin fish
  • Muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Computational Mathematics


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