TY - GEN
T1 - Erythrocytes analysis with a Digital Holographic Microscope
AU - Rappaz, B.
AU - Barbul, A.
AU - Charrière, F.
AU - Kühn, J.
AU - Marquet, P.
AU - Korenstein, R.
AU - Depeursinge, C.
AU - Magistretti, P.
PY - 2007
Y1 - 2007
N2 - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy (Marquet et al., 2005). Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed (Rappaz et al., 2005) a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible, for the first time to our knowledge, to accurately measure (with a precision of 0.0003) the mean refractive index of living erythrocytes.. On the other hand, the cellular thickness measurements allow to calculate the volume and shape of erythrocytes. In addition, DHM, thanks to its subwavelength phase shift measurements, was found to yield an efficient tool to assess erythrocyte cell membrane fluctuations (ECMF). Typically, ECMF characterized by an amplitude within the range of 45 nm were observed.
AB - Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy (Marquet et al., 2005). Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed (Rappaz et al., 2005) a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible, for the first time to our knowledge, to accurately measure (with a precision of 0.0003) the mean refractive index of living erythrocytes.. On the other hand, the cellular thickness measurements allow to calculate the volume and shape of erythrocytes. In addition, DHM, thanks to its subwavelength phase shift measurements, was found to yield an efficient tool to assess erythrocyte cell membrane fluctuations (ECMF). Typically, ECMF characterized by an amplitude within the range of 45 nm were observed.
KW - Cell imaging
KW - Digital holography
KW - Erythrocyte
KW - Refractive index
UR - http://www.scopus.com/inward/record.url?scp=36248954569&partnerID=8YFLogxK
U2 - 10.1364/ecbo.2007.6631_17
DO - 10.1364/ecbo.2007.6631_17
M3 - Conference contribution
AN - SCOPUS:36248954569
SN - 0819467758
SN - 9780819467751
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Novel Optical Instrumentation for Biomedical Applications III
PB - SPIE
T2 - Novel Optical Instrumentation for Biomedical Applications III
Y2 - 17 June 2007 through 19 June 2007
ER -