Enhanced antigen presentation and immunostimulation of dendritic cells using acid-degradable cationic nanoparticles

Young Jik Kwon; Stephany M. Standley; Sarah L. Goh; Jean M.J. Fréchet

doi:10.1016/j.jconrel.2005.02.027

Enhanced antigen presentation and immunostimulation of dendritic cells using acid-degradable cationic nanoparticles

Young Jik Kwon, Stephany M. Standley, Sarah L. Goh, Jean M.J. Fréchet^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

141 Scopus citations

Abstract

Acid-degradable cationic nanoparticles encapsulating a model antigen (i.e., ovalbumin) were prepared by inverse microemulsion polymerization with acid-cleavable acetal cross-linkers. Incubation of these degradable nanoparticles with dendritic cells derived from bone marrow (BMDCs) resulted in the enhanced presentation of ovalbumin-derived peptides, as quantified by B3Z cells, a CD8⁺ T cell hybridoma. The cationic nature of the particles contributed to the increased surface endocytosis (or phagocytosis) observed with BMDCs, which is the first barrier to overcome for successful antigen delivery. The acid sensitivity of the particles served to direct more ovalbumin antigens to be processed into the appropriately trimmed peptide fragments and presented via the major histocompatibility complex (MHC) class I pathway following hydrolysis within the acidic lysosomes. It was also shown that adjuvant molecules such as unmethylated CpG oligonucleotides (CpG ODN) and anti-interleukin-10 oligonucleotides (AS10 ODN) could be co-delivered with the protein antigen for maximized cellular immune response.

Original language	English (US)
Pages (from-to)	199-212
Number of pages	14
Journal	Journal of Controlled Release
Volume	105
Issue number	3
DOIs	https://doi.org/10.1016/j.jconrel.2005.02.027
State	Published - Jul 20 2005
Externally published	Yes

Bibliographical note

Funding Information:
Financial support of portions of this work by the National Institute of Health (Grant RO1EB002047) and, in part, by U.S. Department of Energy (Grant DE-ACO3-76SF00098) for the development of stimuli responsive nanoparticles is acknowledged with thanks.

Keywords

Adjuvant oligonucleotides
Antigen presentation
Cancer vaccine
Degradable nanoparticle
Dendritic cells

ASJC Scopus subject areas

Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jconrel.2005.02.027

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title = "Enhanced antigen presentation and immunostimulation of dendritic cells using acid-degradable cationic nanoparticles",

abstract = "Acid-degradable cationic nanoparticles encapsulating a model antigen (i.e., ovalbumin) were prepared by inverse microemulsion polymerization with acid-cleavable acetal cross-linkers. Incubation of these degradable nanoparticles with dendritic cells derived from bone marrow (BMDCs) resulted in the enhanced presentation of ovalbumin-derived peptides, as quantified by B3Z cells, a CD8+ T cell hybridoma. The cationic nature of the particles contributed to the increased surface endocytosis (or phagocytosis) observed with BMDCs, which is the first barrier to overcome for successful antigen delivery. The acid sensitivity of the particles served to direct more ovalbumin antigens to be processed into the appropriately trimmed peptide fragments and presented via the major histocompatibility complex (MHC) class I pathway following hydrolysis within the acidic lysosomes. It was also shown that adjuvant molecules such as unmethylated CpG oligonucleotides (CpG ODN) and anti-interleukin-10 oligonucleotides (AS10 ODN) could be co-delivered with the protein antigen for maximized cellular immune response.",

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note = "Funding Information: Financial support of portions of this work by the National Institute of Health (Grant RO1EB002047) and, in part, by U.S. Department of Energy (Grant DE-ACO3-76SF00098) for the development of stimuli responsive nanoparticles is acknowledged with thanks.",

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AU - Goh, Sarah L.

AU - Fréchet, Jean M.J.

N1 - Funding Information: Financial support of portions of this work by the National Institute of Health (Grant RO1EB002047) and, in part, by U.S. Department of Energy (Grant DE-ACO3-76SF00098) for the development of stimuli responsive nanoparticles is acknowledged with thanks.

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Enhanced antigen presentation and immunostimulation of dendritic cells using acid-degradable cationic nanoparticles

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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