Engineering virus resistance via CRISPR–Cas systems

Ahmed Mahas; Magdy M. Mahfouz

doi:10.1016/j.coviro.2018.06.002

Engineering virus resistance via CRISPR–Cas systems

Ahmed Mahas, Magdy M. Mahfouz

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants. Rewired CRISPR/Cas9 and CRISPR/Cas13 systems have been used to confer resistance against DNA and RNA viruses, respectively. Here, we discuss recent approaches employing CRISPR/Cas systems to combat viruses in eukaryotes, highlight key challenges, and provide future perspectives. Moreover, we discuss the application of CRISPR/Cas systems in genome-wide screens to identify key host factors for virus infection to enhance our understanding of basic virus biology and to identify and study virus–host interactions.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Current Opinion in Virology
Volume	32
DOIs	https://doi.org/10.1016/j.coviro.2018.06.002
State	Published - Jul 11 2018

Bibliographical note

KAUST Repository Item: Exported on 2021-11-29
Acknowledgements: We would like to thank the members of The Laboratory for Genome Engineering at KAUST for their helpful discussions and critical reading of the manuscript. We wish to thank the Office of Sponsored Research (OSR) and King Abdullah University of Science and Technology (KAUST) for their support (award OSR-2015-CRG4-2647).

ASJC Scopus subject areas

Virology

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10.1016/j.coviro.2018.06.002

Cite this

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title = "Engineering virus resistance via CRISPR–Cas systems",

abstract = "In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants. Rewired CRISPR/Cas9 and CRISPR/Cas13 systems have been used to confer resistance against DNA and RNA viruses, respectively. Here, we discuss recent approaches employing CRISPR/Cas systems to combat viruses in eukaryotes, highlight key challenges, and provide future perspectives. Moreover, we discuss the application of CRISPR/Cas systems in genome-wide screens to identify key host factors for virus infection to enhance our understanding of basic virus biology and to identify and study virus–host interactions.",

author = "Ahmed Mahas and Mahfouz, {Magdy M.}",

note = "KAUST Repository Item: Exported on 2021-11-29 Acknowledgements: We would like to thank the members of The Laboratory for Genome Engineering at KAUST for their helpful discussions and critical reading of the manuscript. We wish to thank the Office of Sponsored Research (OSR) and King Abdullah University of Science and Technology (KAUST) for their support (award OSR-2015-CRG4-2647).",

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N1 - KAUST Repository Item: Exported on 2021-11-29 Acknowledgements: We would like to thank the members of The Laboratory for Genome Engineering at KAUST for their helpful discussions and critical reading of the manuscript. We wish to thank the Office of Sponsored Research (OSR) and King Abdullah University of Science and Technology (KAUST) for their support (award OSR-2015-CRG4-2647).

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AB - In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants. Rewired CRISPR/Cas9 and CRISPR/Cas13 systems have been used to confer resistance against DNA and RNA viruses, respectively. Here, we discuss recent approaches employing CRISPR/Cas systems to combat viruses in eukaryotes, highlight key challenges, and provide future perspectives. Moreover, we discuss the application of CRISPR/Cas systems in genome-wide screens to identify key host factors for virus infection to enhance our understanding of basic virus biology and to identify and study virus–host interactions.

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Engineering virus resistance via CRISPR–Cas systems

Abstract

Bibliographical note

ASJC Scopus subject areas

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