In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in mammals and plants. Rewired CRISPR/Cas9 and CRISPR/Cas13 systems have been used to confer resistance against DNA and RNA viruses, respectively. Here, we discuss recent approaches employing CRISPR/Cas systems to combat viruses in eukaryotes, highlight key challenges, and provide future perspectives. Moreover, we discuss the application of CRISPR/Cas systems in genome-wide screens to identify key host factors for virus infection to enhance our understanding of basic virus biology and to identify and study virus–host interactions.
|Original language||English (US)|
|Number of pages||8|
|Journal||Current Opinion in Virology|
|State||Published - Jul 11 2018|
Bibliographical noteKAUST Repository Item: Exported on 2021-11-29
Acknowledgements: We would like to thank the members of The Laboratory for Genome Engineering at KAUST for their helpful discussions and critical reading of the manuscript. We wish to thank the Office of Sponsored Research (OSR) and King Abdullah University of Science and Technology (KAUST) for their support (award OSR-2015-CRG4-2647).
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