Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato

Manal Tashkandi; Zahir Ali; Fatimah R. Aljedaani; Ashwag Shami; Magdy M. Mahfouz

doi:10.1080/15592324.2018.1525996

Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato

Manal Tashkandi, Zahir Ali, Fatimah R. Aljedaani, Ashwag Shami, Magdy M. Mahfouz

Research output: Contribution to journal › Article › peer-review

196 Scopus citations

Abstract

CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.

Original language	English (US)
Pages (from-to)	e1525996
Journal	Plant Signaling & Behavior
Volume	13
Issue number	10
DOIs	https://doi.org/10.1080/15592324.2018.1525996
State	Published - Oct 5 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): OSR-2015-CRG4-2647
Acknowledgements: We thank the members of the Laboratory for Genome Engineering for their continuous constructive discussions and comments on the manuscript. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2015-CRG4-2647.

Access to Document

10.1080/15592324.2018.1525996

https://repository.kaust.edu.sa/bitstream/10754/626438/1/237735.full.pdf

Cite this

@article{561e6b26a7294750a1be7074181e1dba,

title = "Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato",

abstract = "CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.",

author = "Manal Tashkandi and Zahir Ali and Aljedaani, {Fatimah R.} and Ashwag Shami and Mahfouz, {Magdy M.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): OSR-2015-CRG4-2647 Acknowledgements: We thank the members of the Laboratory for Genome Engineering for their continuous constructive discussions and comments on the manuscript. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2015-CRG4-2647.",

year = "2018",

month = oct,

day = "5",

doi = "10.1080/15592324.2018.1525996",

language = "English (US)",

volume = "13",

pages = "e1525996",

journal = "Plant Signaling & Behavior",

issn = "1559-2324",

publisher = "Landes Bioscience",

number = "10",

}

TY - JOUR

T1 - Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato

AU - Tashkandi, Manal

AU - Ali, Zahir

AU - Aljedaani, Fatimah R.

AU - Shami, Ashwag

AU - Mahfouz, Magdy M.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): OSR-2015-CRG4-2647 Acknowledgements: We thank the members of the Laboratory for Genome Engineering for their continuous constructive discussions and comments on the manuscript. This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2015-CRG4-2647.

PY - 2018/10/5

Y1 - 2018/10/5

N2 - CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.

AB - CRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with the CRISPR/Cas9 system to confer immunity against the Tomato yellow leaf curl virus (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the N. benthamiana and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in N. benthamiana and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.

UR - http://hdl.handle.net/10754/626438

UR - https://www.tandfonline.com/doi/full/10.1080/15592324.2018.1525996

UR - http://www.scopus.com/inward/record.url?scp=85054532455&partnerID=8YFLogxK

U2 - 10.1080/15592324.2018.1525996

DO - 10.1080/15592324.2018.1525996

M3 - Article

C2 - 30289378

SN - 1559-2324

VL - 13

SP - e1525996

JO - Plant Signaling & Behavior

JF - Plant Signaling & Behavior

IS - 10

ER -

Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Engineering resistance against $\textit{Tomato yellow leaf curl virus}$ via the CRISPR/Cas9 system in tomato

Cite this

Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Datasets

Engineering resistance against $\textit{Tomato yellow leaf curl virus}$ via the CRISPR/Cas9 system in tomato

Cite this