Efficient insertional mutagenesis in rice using the maize En/Spm elements

Chellian Santhosh Kumar, Rod A. Wing, Venkatesan Sundaresan

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


We have developed a novel system for insertional mutagenesis in rice (Oryza sativa) based on the maize (Zea mays) enhancer/suppressor mutator (En/Spm) element. In this system, a single T-DNA construct with Spm-transposase and the non-autonomous defective suppressor mutator (dSpm) element is used in conjunction with green fluorescent protein (GFP) and Discosoma sp. Red Fluorescence Protein (DsRed) fluorescent markers to select unlinked stable transpositions of dSpm. Using this system, we could demonstrate high frequencies of unlinked germinal transposition of dSpm in rice. Analysis of dSpm flanking sequences from 353 stable insertion lines revealed that the dSpm insertions appear to be widely distributed on rice chromosomes with a preference for genic regions (70%). The dSpm insertions appear to differ from Activator-Dissociation (Ac-Ds) elements in genomic distribution and exhibit a greater fraction of unlinked transpositions when compared with Ds elements. The results obtained in this study demonstrate that the maize En/Spm element can be used as an effective tool for functional genomics in rice and can complement efforts using other insertional mutagens. Further, the efficacy of the non-invasive fluorescence-based selection system is promising for its application to other crops. © 2005 Blackwell Publishing Ltd.
Original languageEnglish (US)
JournalPlant Journal
Issue number5
StatePublished - Dec 1 2005
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2019-11-20


Dive into the research topics of 'Efficient insertional mutagenesis in rice using the maize En/Spm elements'. Together they form a unique fingerprint.

Cite this