Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain

Nicolas C. Schaad; Pierre J. Magistretti; Michel Schorderet

doi:10.1016/0741-8329(88)90080-8

Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain

Nicolas C. Schaad^*, Pierre J. Magistretti, Michel Schorderet

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as shown in potentiating, at toxic concentrations, the stimulating effect of hormones or neurotransmitters. We have also found that EtOH potentiates in a dose-dependent manner (EC₅₀=100 mM) the cAMP production elicited by vasoactive intestinal peptide (VIP), already noticeably at 70 mM, without affecting basal cAMP levels (up to 400 mM). Propanol produces a similar potentiation, whereas methanol was inactive. Butanol (200 mM) displays toxic effects. The potentiation induced by EtOH is similar for peptide- (VIP) or monoamine- (noradrenaline) stimulated cAMP formation, suggesting a primary action at a level different from the receptor recognition sites. Finally, we have observed that EtOH potentiates the synergistic interaction between VIP and NA in stimulating cAMP formation.

Original language	English (US)
Pages (from-to)	445-449
Number of pages	5
Journal	Alcohol
Volume	5
Issue number	6
DOIs	https://doi.org/10.1016/0741-8329(88)90080-8
State	Published - 1988
Externally published	Yes

Keywords

Adenylate cyclase
Ethanol
Noradrenaline
Vasoactive intestinal peptide

ASJC Scopus subject areas

Health(social science)
Neurology
Biochemistry
Behavioral Neuroscience
Toxicology

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10.1016/0741-8329(88)90080-8

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title = "Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain",

abstract = "Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as shown in potentiating, at toxic concentrations, the stimulating effect of hormones or neurotransmitters. We have also found that EtOH potentiates in a dose-dependent manner (EC50=100 mM) the cAMP production elicited by vasoactive intestinal peptide (VIP), already noticeably at 70 mM, without affecting basal cAMP levels (up to 400 mM). Propanol produces a similar potentiation, whereas methanol was inactive. Butanol (200 mM) displays toxic effects. The potentiation induced by EtOH is similar for peptide- (VIP) or monoamine- (noradrenaline) stimulated cAMP formation, suggesting a primary action at a level different from the receptor recognition sites. Finally, we have observed that EtOH potentiates the synergistic interaction between VIP and NA in stimulating cAMP formation.",

keywords = "Adenylate cyclase, Ethanol, Noradrenaline, Vasoactive intestinal peptide",

author = "Schaad, {Nicolas C.} and Magistretti, {Pierre J.} and Michel Schorderet",

year = "1988",

doi = "10.1016/0741-8329(88)90080-8",

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pages = "445--449",

journal = "Alcohol",

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TY - JOUR

T1 - Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain

AU - Schaad, Nicolas C.

AU - Magistretti, Pierre J.

AU - Schorderet, Michel

PY - 1988

Y1 - 1988

N2 - Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as shown in potentiating, at toxic concentrations, the stimulating effect of hormones or neurotransmitters. We have also found that EtOH potentiates in a dose-dependent manner (EC50=100 mM) the cAMP production elicited by vasoactive intestinal peptide (VIP), already noticeably at 70 mM, without affecting basal cAMP levels (up to 400 mM). Propanol produces a similar potentiation, whereas methanol was inactive. Butanol (200 mM) displays toxic effects. The potentiation induced by EtOH is similar for peptide- (VIP) or monoamine- (noradrenaline) stimulated cAMP formation, suggesting a primary action at a level different from the receptor recognition sites. Finally, we have observed that EtOH potentiates the synergistic interaction between VIP and NA in stimulating cAMP formation.

AB - Among several effects, ethanol (EtOH) interferes with membrane fluidity and lipid-protein interactions. As proteins are influenced by surrounding lipids, the activity of membrane-bound enzymes such as adenylate cyclase (AC) could be modulated by EtOH, as shown in potentiating, at toxic concentrations, the stimulating effect of hormones or neurotransmitters. We have also found that EtOH potentiates in a dose-dependent manner (EC50=100 mM) the cAMP production elicited by vasoactive intestinal peptide (VIP), already noticeably at 70 mM, without affecting basal cAMP levels (up to 400 mM). Propanol produces a similar potentiation, whereas methanol was inactive. Butanol (200 mM) displays toxic effects. The potentiation induced by EtOH is similar for peptide- (VIP) or monoamine- (noradrenaline) stimulated cAMP formation, suggesting a primary action at a level different from the receptor recognition sites. Finally, we have observed that EtOH potentiates the synergistic interaction between VIP and NA in stimulating cAMP formation.

KW - Adenylate cyclase

KW - Ethanol

KW - Noradrenaline

KW - Vasoactive intestinal peptide

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U2 - 10.1016/0741-8329(88)90080-8

DO - 10.1016/0741-8329(88)90080-8

M3 - Article

C2 - 2854474

AN - SCOPUS:0024162764

SN - 0741-8329

VL - 5

SP - 445

EP - 449

JO - Alcohol

JF - Alcohol

IS - 6

ER -

Effects of ethanol on VIP- and/or noradrenaline-stimulated cAMP formation in mouse brain

Abstract

Keywords

ASJC Scopus subject areas

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