Effect of a hyperlipidic diet on lipid composition, fluidity, and (na+-k+)ATPase activity of rat erythrocyte membranes

A. Bordoni, P. L. Biagi, G. Parenti Castelli, S. Hrelia, C. A. Rossi, G. Lercker, J. C. Izpisua, T. Barber, J. Cabo, G. Lenaz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Feeding rats a hyperlipidic diet in which animals were offered daily a variety of high-energy food resulted in a significant increase of serum free fatty acids and a decrease of phospholipids with respect to controls. On the contrary, there were no significant differences in erythrocyte membrane total lipid composition between the two groups. Erythrocyte membranes showed a significant decrease in saturated fatty acid content and a significant increase in (n-6) polyunsaturated fatty acid content; (n-3) polyunsaturated fatty acids significantly decreased. Membrane fluidity, investigated by fluorescence polarization of diphenylhexatriene, significantly increased in the erythrocyte membranes of the experimental group. These results seem compatible with the decreased saturated/unsaturated fatty acid ratio. A significant decrease of (Na+-K+)ATPase activity occurred in erythrocyte membranes of the experimental group rats with respect to the controls.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalMolecular Membrane Biology
Issue number1
StatePublished - 1989
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Fondo de Investigaciones sanitarias de la Seguridad Social (Minis-terio de Sanidad y Consumo), Spain, and by Italian Consiglio Nazionale delle Ricerche (C.N.R.), Rome and M.P.I. 60%.


  • Erythrocyte membrane
  • Hyperlipidic diet
  • Lipid composition
  • Membrane fluidity

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Effect of a hyperlipidic diet on lipid composition, fluidity, and (na+-k+)ATPase activity of rat erythrocyte membranes'. Together they form a unique fingerprint.

Cite this