Effect of 6-hydroxydopamine lesions on norepinephrine-induced [3H]glycogen hydrolysis in mouse cortical slices

Pierre J. Magistretti; John H. Morrison; William J. Shoemaker; Floyd E. Bloom

doi:10.1016/0006-8993(83)91297-0

Effect of 6-hydroxydopamine lesions on norepinephrine-induced [³H]glycogen hydrolysis in mouse cortical slices

Pierre J. Magistretti^*, John H. Morrison, William J. Shoemaker, Floyd E. Bloom

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The effects of norepinephrine (NE) on in vitro [³H]glycogenolysis were assessed in slices of cerebral cortex from mice whose cortical noradrenergic innervation had been severely reduced by intracisternal 6-hydroxydopamine (6-OHDA) injections. A supersensitive response to NE was observed, as demonstrated by a decrease in the EC₅₀ of the catecholamine in the lesioned mice from 533 ± 88 nM to 39.3 ± 7.9 nM. This supersensitive response, observed two weeks after the lesion, was post-synaptic since isoproterenol, a β-adrenergic agonist not accumulated by pre-synaptic uptake mechanisms, also gave an equally supersensitive response.

Original language	English (US)
Pages (from-to)	159-162
Number of pages	4
Journal	Brain Research
Volume	261
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)91297-0
State	Published - Feb 14 1983
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Ms. Lynne Randolph and Ms. Cathy Cannon for skillful technical assistance, and Ms. Nancy Callahan for typing the manuscript. This work was supported by grants from NIMH 29466. P.J.M. was supported by Swiss National Science Foundation Fellowship 83.832.0.80.

Keywords

adrenergic receptors
brain energy metabolism
cerebral cortex
cyclic-AMP
glycogen
supersensitivity

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(83)91297-0

Cite this

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title = "Effect of 6-hydroxydopamine lesions on norepinephrine-induced [3H]glycogen hydrolysis in mouse cortical slices",

abstract = "The effects of norepinephrine (NE) on in vitro [3H]glycogenolysis were assessed in slices of cerebral cortex from mice whose cortical noradrenergic innervation had been severely reduced by intracisternal 6-hydroxydopamine (6-OHDA) injections. A supersensitive response to NE was observed, as demonstrated by a decrease in the EC50 of the catecholamine in the lesioned mice from 533 ± 88 nM to 39.3 ± 7.9 nM. This supersensitive response, observed two weeks after the lesion, was post-synaptic since isoproterenol, a β-adrenergic agonist not accumulated by pre-synaptic uptake mechanisms, also gave an equally supersensitive response.",

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author = "Magistretti, {Pierre J.} and Morrison, {John H.} and Shoemaker, {William J.} and Bloom, {Floyd E.}",

note = "Funding Information: The authors would like to thank Ms. Lynne Randolph and Ms. Cathy Cannon for skillful technical assistance, and Ms. Nancy Callahan for typing the manuscript. This work was supported by grants from NIMH 29466. P.J.M. was supported by Swiss National Science Foundation Fellowship 83.832.0.80.",

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AU - Magistretti, Pierre J.

AU - Morrison, John H.

AU - Shoemaker, William J.

AU - Bloom, Floyd E.

N1 - Funding Information: The authors would like to thank Ms. Lynne Randolph and Ms. Cathy Cannon for skillful technical assistance, and Ms. Nancy Callahan for typing the manuscript. This work was supported by grants from NIMH 29466. P.J.M. was supported by Swiss National Science Foundation Fellowship 83.832.0.80.

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N2 - The effects of norepinephrine (NE) on in vitro [3H]glycogenolysis were assessed in slices of cerebral cortex from mice whose cortical noradrenergic innervation had been severely reduced by intracisternal 6-hydroxydopamine (6-OHDA) injections. A supersensitive response to NE was observed, as demonstrated by a decrease in the EC50 of the catecholamine in the lesioned mice from 533 ± 88 nM to 39.3 ± 7.9 nM. This supersensitive response, observed two weeks after the lesion, was post-synaptic since isoproterenol, a β-adrenergic agonist not accumulated by pre-synaptic uptake mechanisms, also gave an equally supersensitive response.

AB - The effects of norepinephrine (NE) on in vitro [3H]glycogenolysis were assessed in slices of cerebral cortex from mice whose cortical noradrenergic innervation had been severely reduced by intracisternal 6-hydroxydopamine (6-OHDA) injections. A supersensitive response to NE was observed, as demonstrated by a decrease in the EC50 of the catecholamine in the lesioned mice from 533 ± 88 nM to 39.3 ± 7.9 nM. This supersensitive response, observed two weeks after the lesion, was post-synaptic since isoproterenol, a β-adrenergic agonist not accumulated by pre-synaptic uptake mechanisms, also gave an equally supersensitive response.

KW - adrenergic receptors

KW - brain energy metabolism

KW - cerebral cortex

KW - cyclic-AMP

KW - glycogen

KW - supersensitivity

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