Dynamic 15N{1H} NOE measurements: a tool for studying protein dynamics

Vladlena Kharchenko, Michal Nowakowski, Mariusz Jaremko, Andrzej Ejchart, Lukasz Jaremko

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Abstract Intramolecular motions in proteins are one of the important factors that determine their biological activity and interactions with molecules of biological importance. Magnetic relaxation of 15N amide nuclei allows one to monitor motions of protein backbone over a wide range of time scales. 15N{1H} nuclear Overhauser effect is essential for the identification of fast backbone motions in proteins. Therefore, exact measurements of NOE values and their accuracies are critical for determining the picosecond time scale of protein backbone. Measurement of dynamic NOE allows for the determination of NOE values and their probable errors defined by any sound criterion of nonlinear regression methods. The dynamic NOE measurements can be readily applied for non-deuterated or deuterated proteins in both HSQC and TROSY-type experiments. Comparison of the dynamic NOE method with commonly implied steady-state NOE is presented in measurements performed at three magnetic field strengths. It is also shown that improperly set NOE measurement cannot be restored with correction factors reported in the literature.
Original languageEnglish (US)
JournalJournal of Biomolecular NMR
StatePublished - Sep 12 2020

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): OSR-CRG2018-3792
Acknowledgements: The authors would like to thank the Imaging and Characterization Core Lab at the King Abdullah University of Science and Technology (KAUST) for the access to the NMR facilities. This publication is based upon work supported by KAUST Office of Sponsored Research (OSR) under Award No. OSR-CRG2018-3792 (LJ) and through baseline-funds (LJ and MJ).


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