Do-it-yourself guide: How to use the modern single-molecule toolkit

Nils G. Walter*, Cheng Yen Huang, Anthony J. Manzo, Mohamed A. Sobhy

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

276 Scopus citations


Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethered particle microscopy, optical and magnetic tweezers, and atomic force microscopy. We also provide guidelines for choosing the right approach from the available single-molecule toolkit for applications as diverse as structural biology, enzymology, nanotechnology and systems biology.

Original languageEnglish (US)
Pages (from-to)475-489
Number of pages15
JournalNature Methods
Issue number6
StatePublished - Jun 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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