DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

Peigang Deng; Donald C. Chang; Yi-Kuen Lee; Junwei Zhou; Gang Li

doi:10.1109/nems.2011.6017304

DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

Peigang Deng, Donald C. Chang, Yi-Kuen Lee, Junwei Zhou, Gang Li

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Scopus citations

Abstract

Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.

Original language	English (US)
Title of host publication	2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	96-99
Number of pages	4
ISBN (Print)	9781612847757
DOIs	https://doi.org/10.1109/nems.2011.6017304
State	Published - Feb 2011

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040, UK-COOI6
Acknowledgements: This research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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10.1109/nems.2011.6017304

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@inproceedings{f5adfa64e3f14bb59e1b2916062c719d,

title = "DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips",

abstract = "Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP {"}phase diagram{"}, which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP {"}phase diagram{"}. {\textcopyright} 2011 IEEE.",

author = "Peigang Deng and Chang, {Donald C.} and Yi-Kuen Lee and Junwei Zhou and Gang Li",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040, UK-COOI6 Acknowledgements: This research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907). This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2011",

month = feb,

doi = "10.1109/nems.2011.6017304",

language = "English (US)",

isbn = "9781612847757",

pages = "96--99",

booktitle = "2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

}

DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips. / Deng, Peigang; Chang, Donald C.; Lee, Yi-Kuen et al.
2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Institute of Electrical and Electronics Engineers (IEEE), 2011. p. 96-99.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

AU - Deng, Peigang

AU - Chang, Donald C.

AU - Lee, Yi-Kuen

AU - Zhou, Junwei

AU - Li, Gang

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): SA-C0040, UK-COOI6 Acknowledgements: This research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907). This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2011/2

Y1 - 2011/2

N2 - Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.

AB - Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.

UR - http://hdl.handle.net/10754/600249

UR - http://ieeexplore.ieee.org/document/6017304/

UR - http://www.scopus.com/inward/record.url?scp=80053337098&partnerID=8YFLogxK

U2 - 10.1109/nems.2011.6017304

DO - 10.1109/nems.2011.6017304

M3 - Conference contribution

SN - 9781612847757

SP - 96

EP - 99

BT - 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

PB - Institute of Electrical and Electronics Engineers (IEEE)

ER -

DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

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