Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Besma Ettoumi; Habib Chouchane; Amel Guesmi; Mouna Mahjoubi; Lorenzo Brusetti; Mohamed Neifar; Sara Borin; Daniele Daffonchio; Ameur Cherif

doi:10.1016/j.micres.2016.03.006

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Besma Ettoumi, Habib Chouchane, Amel Guesmi, Mouna Mahjoubi, Lorenzo Brusetti, Mohamed Neifar, Sara Borin, Daniele Daffonchio, Ameur Cherif

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.

Original language	English (US)
Pages (from-to)	71-80
Number of pages	10
Journal	Microbiological Research
Volume	186-187
DOIs	https://doi.org/10.1016/j.micres.2016.03.006
State	Published - Apr 3 2016

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un-
Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr.
Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews
of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his
help in samples collection. This work was partly supported by the Ministry of High Education
and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the
European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under
grant agreement no. 266473).

ASJC Scopus subject areas

Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micres.2016.03.006

https://repository.kaust.edu.sa/bitstream/10754/604744/1/1-s2.0-S0944501316300817-main.pdf

Cite this

@article{90bd9f65ee00485295f1cc130d0f0119,

title = "Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea",

abstract = "In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.",

author = "Besma Ettoumi and Habib Chouchane and Amel Guesmi and Mouna Mahjoubi and Lorenzo Brusetti and Mohamed Neifar and Sara Borin and Daniele Daffonchio and Ameur Cherif",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un- Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr. Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his help in samples collection. This work was partly supported by the Ministry of High Education and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under grant agreement no. 266473).",

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doi = "10.1016/j.micres.2016.03.006",

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T1 - Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

AU - Ettoumi, Besma

AU - Chouchane, Habib

AU - Guesmi, Amel

AU - Mahjoubi, Mouna

AU - Brusetti, Lorenzo

AU - Neifar, Mohamed

AU - Borin, Sara

AU - Daffonchio, Daniele

AU - Cherif, Ameur

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un- Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr. Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his help in samples collection. This work was partly supported by the Ministry of High Education and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under grant agreement no. 266473).

PY - 2016/4/3

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N2 - In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.

AB - In the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.

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U2 - 10.1016/j.micres.2016.03.006

DO - 10.1016/j.micres.2016.03.006

M3 - Article

C2 - 27242145

SN - 0944-5013

VL - 186-187

SP - 71

EP - 80

JO - Microbiological Research

JF - Microbiological Research

ER -

Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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