Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene

Eva Janáková; Irena Jakobson; Hilma Peusha; Michael Abrouk; Monika Škopová; Hana Šimková; Jan Šafář; Jan Vrána; Jaroslav Doležel; Kadri Järve; Miroslav Valárik

doi:10.1007/s00122-018-3259-3

Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene

Eva Janáková, Irena Jakobson, Hilma Peusha, Michael Abrouk, Monika Škopová, Hana Šimková, Jan Šafář, Jan Vrána, Jaroslav Doležel, Kadri Järve, Miroslav Valárik

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments’ sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.

Original language	English (US)
Pages (from-to)	1061-1072
Number of pages	12
Journal	Theoretical and Applied Genetics
Volume	132
Issue number	4
DOIs	https://doi.org/10.1007/s00122-018-3259-3
State	Published - Dec 7 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The technical assistance of Marie Seifertová, Radka Tušková, Eva Jahnová and Helena Tvardíková is highly appreciated. This research was financially supported by the Czech Republic Ministry of Education, Youth and Sports (Award LO1204 from the National Program of Sustainability I), by the Czech Science Foundation (Award 18-11688S), the Czech Republic Ministry of Agriculture (Award QK1710302) and by an IUT 193 Grant from the Estonian Ministry of Education and Research. The authors thank the International Wheat Genome Sequencing Consortium (IWGSC) for pre-publication access to IWGSC RefSeq v1.0, the National BioResource Centre (Kyoto, Japan) for providing the CS aneuploid stocks and S. Reader (John Innes Centre, Norwich, UK) for the gift of grain of the ph1b mutant. Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the program “Projects of Large Research, Development and Innovation Infrastructures” (CESNET LM2015042) is greatly appreciated.

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https://repository.kaust.edu.sa/bitstream/10754/630263/1/Jan%c3%a1kov%c3%a12018_Article_DivergenceBetweenBreadWheatAnd.pdf

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Janáková, E., Jakobson, I., Peusha, H., Abrouk, M., Škopová, M., Šimková, H., Šafář, J., Vrána, J., Doležel, J., Järve, K., & Valárik, M. (2018). Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene. Theoretical and Applied Genetics, 132(4), 1061-1072. https://doi.org/10.1007/s00122-018-3259-3

@article{14770bc8724945c5be2753d750c5afbf,

title = "Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene",

abstract = "A segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments{\textquoteright} sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.",

author = "Eva Jan{\'a}kov{\'a} and Irena Jakobson and Hilma Peusha and Michael Abrouk and Monika {\v S}kopov{\'a} and Hana {\v S}imkov{\'a} and Jan {\v S}af{\'a}{\v r} and Jan Vr{\'a}na and Jaroslav Dole{\v z}el and Kadri J{\"a}rve and Miroslav Val{\'a}rik",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The technical assistance of Marie Seifertov{\'a}, Radka Tu{\v s}kov{\'a}, Eva Jahnov{\'a} and Helena Tvard{\'i}kov{\'a} is highly appreciated. This research was financially supported by the Czech Republic Ministry of Education, Youth and Sports (Award LO1204 from the National Program of Sustainability I), by the Czech Science Foundation (Award 18-11688S), the Czech Republic Ministry of Agriculture (Award QK1710302) and by an IUT 193 Grant from the Estonian Ministry of Education and Research. The authors thank the International Wheat Genome Sequencing Consortium (IWGSC) for pre-publication access to IWGSC RefSeq v1.0, the National BioResource Centre (Kyoto, Japan) for providing the CS aneuploid stocks and S. Reader (John Innes Centre, Norwich, UK) for the gift of grain of the ph1b mutant. Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the program “Projects of Large Research, Development and Innovation Infrastructures” (CESNET LM2015042) is greatly appreciated.",

year = "2018",

month = dec,

day = "7",

doi = "10.1007/s00122-018-3259-3",

language = "English (US)",

volume = "132",

pages = "1061--1072",

journal = "Theoretical and Applied Genetics",

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Janáková, E, Jakobson, I, Peusha, H, Abrouk, M, Škopová, M, Šimková, H, Šafář, J, Vrána, J, Doležel, J, Järve, K & Valárik, M 2018, 'Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene', Theoretical and Applied Genetics, vol. 132, no. 4, pp. 1061-1072. https://doi.org/10.1007/s00122-018-3259-3

TY - JOUR

T1 - Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene

AU - Janáková, Eva

AU - Jakobson, Irena

AU - Peusha, Hilma

AU - Abrouk, Michael

AU - Škopová, Monika

AU - Šimková, Hana

AU - Šafář, Jan

AU - Vrána, Jan

AU - Doležel, Jaroslav

AU - Järve, Kadri

AU - Valárik, Miroslav

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The technical assistance of Marie Seifertová, Radka Tušková, Eva Jahnová and Helena Tvardíková is highly appreciated. This research was financially supported by the Czech Republic Ministry of Education, Youth and Sports (Award LO1204 from the National Program of Sustainability I), by the Czech Science Foundation (Award 18-11688S), the Czech Republic Ministry of Agriculture (Award QK1710302) and by an IUT 193 Grant from the Estonian Ministry of Education and Research. The authors thank the International Wheat Genome Sequencing Consortium (IWGSC) for pre-publication access to IWGSC RefSeq v1.0, the National BioResource Centre (Kyoto, Japan) for providing the CS aneuploid stocks and S. Reader (John Innes Centre, Norwich, UK) for the gift of grain of the ph1b mutant. Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum provided under the program “Projects of Large Research, Development and Innovation Infrastructures” (CESNET LM2015042) is greatly appreciated.

PY - 2018/12/7

Y1 - 2018/12/7

N2 - A segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments’ sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.

AB - A segment of Triticum militinae chromosome 7G harbors a gene(s) conferring powdery mildew resistance which is effective at both the seedling and the adult plant stages when transferred into bread wheat (T. aestivum). The introgressed segment replaces a piece of wheat chromosome arm 4AL. An analysis of segregating materials generated to positionally clone the gene highlighted that in a plant heterozygous for the introgression segment, only limited recombination occurs between the introgressed region and bread wheat 4A. Nevertheless, 75 genetic markers were successfully placed within the region, thereby confining the gene to a 0.012 cM window along the 4AL arm. In a background lacking the Ph1 locus, the localized rate of recombination was raised 33-fold, enabling the reduction in the length of the region containing the resistance gene to a 480 kbp stretch harboring 12 predicted genes. The substituted segment in the reference sequence of bread wheat cv. Chinese Spring is longer (640 kbp) and harbors 16 genes. A comparison of the segments’ sequences revealed a high degree of divergence with respect to both their gene content and nucleotide sequence. Of the 12 T. militinae genes, only four have a homolog in cv. Chinese Spring. Possible candidate genes for the resistance have been identified based on function predicted from their sequence.

UR - http://hdl.handle.net/10754/630263

UR - https://link.springer.com/article/10.1007%2Fs00122-018-3259-3

UR - http://www.scopus.com/inward/record.url?scp=85058098454&partnerID=8YFLogxK

U2 - 10.1007/s00122-018-3259-3

DO - 10.1007/s00122-018-3259-3

M3 - Article

C2 - 30535646

SN - 0040-5752

VL - 132

SP - 1061

EP - 1072

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

IS - 4

ER -

Divergence between bread wheat and Triticum militinae in the powdery mildew resistance QPm.tut-4A locus and its implications for cloning of the resistance gene

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