TY - JOUR
T1 - Distribution of alkane-degrading bacterial communities in soils from King George Island, Maritime Antarctic
AU - Jurelevicius, Diogo
AU - Cotta, Simone Raposo
AU - Peixoto, Raquel
AU - Rosado, Alexandre Soares
AU - Seldin, Lucy
N1 - Generated from Scopus record by KAUST IRTS on 2021-02-16
PY - 2012/7/1
Y1 - 2012/7/1
N2 - The structure of alkane-degrading bacterial communities, which are present in both the hydrocarbon-polluted and pristine soils of King George Island in Maritime Antarctic, was studied using molecular methods. Polymerase Chain Reaction (PCR) amplifications of the alkane monooxygenase AlkB-coding genes, followed by Restriction Fragment Length Polymorphism (PCR-RFLP) and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analyses, revealed the widespread presence and complex diversity of alkane-utilizing bacteria in these soils. The resulting dendrograms and Canonical Correspondence Analyses (CCA) of PCR-RFLP and PCR-DGGE patterns showed that the characteristics of the different soils, such as physicochemical properties, soil type and/or hydrocarbon contamination levels, affect the distribution of alkane-degrading bacteria. Sequencing of 20 DGGE bands revealed the presence in Antarctic soils of alkane monooxygenases with low similarity (61-91%) compared to those previously described in Gram-positive bacteria, such as Mycobacterium, Gordonia, Rhodococcus and Aeromicrobium. The high diversity of alkB genes in the soils of King George Island suggests the potential for oil pollutant degradation. © 2012 Elsevier Masson SAS.
AB - The structure of alkane-degrading bacterial communities, which are present in both the hydrocarbon-polluted and pristine soils of King George Island in Maritime Antarctic, was studied using molecular methods. Polymerase Chain Reaction (PCR) amplifications of the alkane monooxygenase AlkB-coding genes, followed by Restriction Fragment Length Polymorphism (PCR-RFLP) and Denaturing Gradient Gel Electrophoresis (PCR-DGGE) analyses, revealed the widespread presence and complex diversity of alkane-utilizing bacteria in these soils. The resulting dendrograms and Canonical Correspondence Analyses (CCA) of PCR-RFLP and PCR-DGGE patterns showed that the characteristics of the different soils, such as physicochemical properties, soil type and/or hydrocarbon contamination levels, affect the distribution of alkane-degrading bacteria. Sequencing of 20 DGGE bands revealed the presence in Antarctic soils of alkane monooxygenases with low similarity (61-91%) compared to those previously described in Gram-positive bacteria, such as Mycobacterium, Gordonia, Rhodococcus and Aeromicrobium. The high diversity of alkB genes in the soils of King George Island suggests the potential for oil pollutant degradation. © 2012 Elsevier Masson SAS.
UR - https://linkinghub.elsevier.com/retrieve/pii/S1164556312000313
UR - http://www.scopus.com/inward/record.url?scp=84859799112&partnerID=8YFLogxK
U2 - 10.1016/j.ejsobi.2012.03.006
DO - 10.1016/j.ejsobi.2012.03.006
M3 - Article
SN - 1164-5563
VL - 51
SP - 37
EP - 44
JO - European Journal of Soil Biology
JF - European Journal of Soil Biology
ER -