Discovery of Resistance Genes in Rye by Targeted Long-Read Sequencing and Association Genetics

Nikolaj M. Vendelbo*, Khalid Mahmood, Burkhard Steuernagel, Brande B.H. Wulff, Pernille Sarup, Mogens S. Hovmøller, Annemarie Fejer Justesen, Peter S. Kristensen, Jihad Orabi, Ahmed Jahoor

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The majority of released rye cultivars are susceptible to leaf rust because of a low level of resistance in the predominant hybrid rye-breeding gene pools Petkus and Carsten. To discover new sources of leaf rust resistance, we phenotyped a diverse panel of inbred lines from the less prevalent Gülzow germplasm using six distinct isolates of Puccinia recondita f. sp. secalis and found that 55 out of 92 lines were resistant to all isolates. By performing a genome-wide association study using 261,406 informative SNP markers, we identified five resistance-associated QTLs on chromosome arms 1RS, 1RL, 2RL, 5RL and 7RS. To identify candidate Puccinia recondita (Pr) resistance genes in these QTLs, we sequenced the rye nucleotide-binding leucine-rich repeat (NLR) intracellular immune receptor complement using a Triticeae NLR bait-library and PacBio® long-read single-molecule high-fidelity (HiFi) sequencing. Trait-genotype correlations across 10 resistant and 10 susceptible lines identified four candidate NLR-encoding Pr genes. One of these physically co-localized with molecular markers delimiting Pr3 on chromosome arm 1RS and the top-most resistance-associated QTL in the panel.

Original languageEnglish (US)
Article number1273
JournalCells
Volume11
Issue number8
DOIs
StatePublished - Apr 1 2022

Bibliographical note

Funding Information:
Funding: The research was funded by Innovation Fund Denmark (grant no. 8053-00085B), the Pajbjerg Foundation, the Danish Green Development and Demonstration Program (project Jr. NR. 34009-19-1603), and the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme (BBS/E/J/000PR9780), and King Abdullah University of Science and Technology.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • brown rust
  • genome-wide association study (GWAS)
  • k-mer
  • leaf rust
  • nucleotide-binding leucine-rich repeat (NLR)
  • Puccinia recondita f. sp. secalis
  • resistance gene enrichment sequencing (RenSeq)
  • Secale cereale L
  • single-molecule high-fidelity sequencing (HiFi)

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Discovery of Resistance Genes in Rye by Targeted Long-Read Sequencing and Association Genetics'. Together they form a unique fingerprint.

Cite this