Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds

Shelley A. Claridge; Sarah L. Goh; Jean M.J. Fréchet; Shara C. Williams; Christine M. Micheel; A. Paul Alivisatos

doi:10.1021/cm0484089

Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds

Shelley A. Claridge, Sarah L. Goh, Jean M.J. Fréchet, Shara C. Williams, Christine M. Micheel, A. Paul Alivisatos

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

The concept of self-assembled dendrimers is explored for the creation of discrete nanoparticle assemblies. Hybridization of branched DNA trimers and nanoparticle-DNA conjugates results in the synthesis of nanoparticle trimer and tetramer complexes. Multiple tetramer architectures are investigated, utilizing Au-DNA conjugates with varying secondary structural motifs. Hybridization products are analyzed by gel electrophoresis, and discrete bands are observed corresponding to structures with increasing numbers of hybridization events. Samples extracted from each band are analyzed by transmission electron microscopy, and statistics compiled from micrographs are used to compare assembly characteristics for each architecture. Asymmetric structures are also produced in which both 5- and 10-nm Au particles are assembled on branched scaffolds.

Original language	English (US)
Pages (from-to)	1628-1635
Number of pages	8
Journal	Chemistry of Materials
Volume	17
Issue number	7
DOIs	https://doi.org/10.1021/cm0484089
State	Published - Apr 5 2005
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering
Materials Chemistry

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10.1021/cm0484089

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title = "Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds",

abstract = "The concept of self-assembled dendrimers is explored for the creation of discrete nanoparticle assemblies. Hybridization of branched DNA trimers and nanoparticle-DNA conjugates results in the synthesis of nanoparticle trimer and tetramer complexes. Multiple tetramer architectures are investigated, utilizing Au-DNA conjugates with varying secondary structural motifs. Hybridization products are analyzed by gel electrophoresis, and discrete bands are observed corresponding to structures with increasing numbers of hybridization events. Samples extracted from each band are analyzed by transmission electron microscopy, and statistics compiled from micrographs are used to compare assembly characteristics for each architecture. Asymmetric structures are also produced in which both 5- and 10-nm Au particles are assembled on branched scaffolds.",

author = "Claridge, {Shelley A.} and Goh, {Sarah L.} and Fr{\'e}chet, {Jean M.J.} and Williams, {Shara C.} and Micheel, {Christine M.} and Alivisatos, {A. Paul}",

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AU - Claridge, Shelley A.

AU - Goh, Sarah L.

AU - Fréchet, Jean M.J.

AU - Williams, Shara C.

AU - Micheel, Christine M.

AU - Alivisatos, A. Paul

PY - 2005/4/5

Y1 - 2005/4/5

N2 - The concept of self-assembled dendrimers is explored for the creation of discrete nanoparticle assemblies. Hybridization of branched DNA trimers and nanoparticle-DNA conjugates results in the synthesis of nanoparticle trimer and tetramer complexes. Multiple tetramer architectures are investigated, utilizing Au-DNA conjugates with varying secondary structural motifs. Hybridization products are analyzed by gel electrophoresis, and discrete bands are observed corresponding to structures with increasing numbers of hybridization events. Samples extracted from each band are analyzed by transmission electron microscopy, and statistics compiled from micrographs are used to compare assembly characteristics for each architecture. Asymmetric structures are also produced in which both 5- and 10-nm Au particles are assembled on branched scaffolds.

AB - The concept of self-assembled dendrimers is explored for the creation of discrete nanoparticle assemblies. Hybridization of branched DNA trimers and nanoparticle-DNA conjugates results in the synthesis of nanoparticle trimer and tetramer complexes. Multiple tetramer architectures are investigated, utilizing Au-DNA conjugates with varying secondary structural motifs. Hybridization products are analyzed by gel electrophoresis, and discrete bands are observed corresponding to structures with increasing numbers of hybridization events. Samples extracted from each band are analyzed by transmission electron microscopy, and statistics compiled from micrographs are used to compare assembly characteristics for each architecture. Asymmetric structures are also produced in which both 5- and 10-nm Au particles are assembled on branched scaffolds.

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IS - 7

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Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds

Abstract

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