Direct Visualization and Identification of Membrane Voltage-Gated Sodium Channels from Human iPSC-Derived Neurons by Multiple Imaging and Light Enhanced Spectroscopy

Manola Moretti, Tania Limongi, Claudia Testi, Edoardo Milanetti, Maria Teresa De Angelis, Elvira I Parrotta, Stefania Scalise, Gianluca Santamaria, Marco Allione, Sergei Lopatin, Bruno Torre, Peng Zhang, Monica Marini, Gerardo Perozziello, Patrizio Candeloro, Candido Fabrizio Pirri, Giancarlo Ruocco, Giovanni Cuda, Enzo Di Fabrizio

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, transmission electron microscopy atomic force microscopy, and surface enhanced Raman spectroscopy are combined through a direct imaging approach, to gather structural and chemical information of complex molecular systems such as ion channels in their original plasma membrane. Customized microfabricated sample holder allows to characterize Nav channels embedded in the original plasma membrane extracted from neuronal cells that are derived from healthy human induced pluripotent stem cells. The identification of the channels is accomplished by using two different approaches, one of them widely used in cryo-EM (the particle analysis method) and the other based on a novel Zernike Polynomial expansion of the images bitmap. This approach allows to carry out a whole series of investigations, one complementary to the other, on the same sample, preserving its state as close as possible to the original membrane configuration.
Original languageEnglish (US)
Pages (from-to)2200402
JournalSmall Methods
DOIs
StatePublished - May 20 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-05-23
Acknowledged KAUST grant number(s): OCRF-2014-CRG, OCRF-2016-CRG
Acknowledgements: The authors acknowledge financial support from King Abdullah University of Science and Technology for OCRF-2014-CRG and OCRF-2016-CRG grants; SHAHEEN cluster to provide machine time; Professor Charlotte A. E. Hauser for providing access to SHAHEEN, and from Piedmont Region through European Funds for Regional Development (“Food Digital Monitoring” project); European Research Council Synergy grant ASTRA (n. 855923).

Fingerprint

Dive into the research topics of 'Direct Visualization and Identification of Membrane Voltage-Gated Sodium Channels from Human iPSC-Derived Neurons by Multiple Imaging and Light Enhanced Spectroscopy'. Together they form a unique fingerprint.

Cite this