Direct imaging of DNA fibers: The visage of double helix

Francesco Gentile; Manola Moretti; Tania Limongi; Andrea Falqui; Giovanni Bertoni; Alice Scarpellini; Stefania Santoriello; Luca Maragliano; Remo Proietti Zaccaria; Enzo Di Fabrizio

doi:10.1021/nl3039162

Direct imaging of DNA fibers: The visage of double helix

Francesco Gentile, Manola Moretti, Tania Limongi, Andrea Falqui, Giovanni Bertoni, Alice Scarpellini, Stefania Santoriello, Luca Maragliano, Remo Proietti Zaccaria, Enzo Di Fabrizio^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.

Original language	English (US)
Pages (from-to)	6453-6458
Number of pages	6
Journal	Nano Letters
Volume	12
Issue number	12
DOIs	https://doi.org/10.1021/nl3039162
State	Published - Dec 12 2012
Externally published	Yes

Keywords

DNA bundles direct imaging
molecular dynamics simulations
nanofabricated micropillars
superhydrophobic surface
transmission electron microscopy

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl3039162

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title = "Direct imaging of DNA fibers: The visage of double helix",

abstract = "Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.",

keywords = "DNA bundles direct imaging, molecular dynamics simulations, nanofabricated micropillars, superhydrophobic surface, transmission electron microscopy",

author = "Francesco Gentile and Manola Moretti and Tania Limongi and Andrea Falqui and Giovanni Bertoni and Alice Scarpellini and Stefania Santoriello and Luca Maragliano and {Proietti Zaccaria}, Remo and {Di Fabrizio}, Enzo",

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doi = "10.1021/nl3039162",

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T1 - Direct imaging of DNA fibers

T2 - The visage of double helix

AU - Gentile, Francesco

AU - Moretti, Manola

AU - Limongi, Tania

AU - Falqui, Andrea

AU - Bertoni, Giovanni

AU - Scarpellini, Alice

AU - Santoriello, Stefania

AU - Maragliano, Luca

AU - Proietti Zaccaria, Remo

AU - Di Fabrizio, Enzo

PY - 2012/12/12

Y1 - 2012/12/12

N2 - Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.

AB - Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.

KW - DNA bundles direct imaging

KW - molecular dynamics simulations

KW - nanofabricated micropillars

KW - superhydrophobic surface

KW - transmission electron microscopy

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SP - 6453

EP - 6458

JO - Nano Letters

JF - Nano Letters

IS - 12

ER -

Direct imaging of DNA fibers: The visage of double helix

Abstract

Keywords

ASJC Scopus subject areas

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