Direct imaging of DNA fibers: The visage of double helix

Francesco Gentile, Manola Moretti, Tania Limongi, Andrea Falqui, Giovanni Bertoni, Alice Scarpellini, Stefania Santoriello, Luca Maragliano, Remo Proietti Zaccaria, Enzo Di Fabrizio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.

Original languageEnglish (US)
Pages (from-to)6453-6458
Number of pages6
JournalNano Letters
Issue number12
StatePublished - Dec 12 2012


  • DNA bundles direct imaging
  • molecular dynamics simulations
  • nanofabricated micropillars
  • superhydrophobic surface
  • transmission electron microscopy

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


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