Direct electrochemistry of cytochrome c immobilized on a graphene oxide-carbon nanotube composite for picomolar detection of hydrogen peroxide

Bose Dinesh, Veerappan Mani, Ramiah Saraswathi, Shen Ming Chen

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

We describe the fabrication of an amperometric biosensor based on cytochrome c (Cyt c) immobilized graphene oxide-multiwalled carbon nanotube (GO-MWCNT) composite on a nano Au modified glassy carbon electrode for trace level detection of H2O2. Morphology and surface characterization of the nanocomposite reveal the successful formation of a highly conducting MWCNT network on the GO surface. Electrochemical impedance studies indicate a lower charge transfer resistance compared to the bare electrode. Cyclic voltammetry studies clearly demonstrate an enhanced direct electrochemistry of Cyt c with a high electron transfer rate constant (k s) value of 3.4 s-1. An amperometric H2O 2 biosensor has been fabricated with an excellent current sensitivity of 0.533 μA pM-1 cm-2 and a very low detection limit of 27.7 pM. The fabricated sensor shows exceptional selectivity to H 2O2 in the presence of a high concentration of some likely interferents. Moreover, the sensor exhibits high stability with appreciable repeatability and reproducibility. © 2014 the Partner Organisations.
Original languageEnglish (US)
Pages (from-to)28229-28237
Number of pages9
JournalRSC ADVANCES
Volume4
Issue number54
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-21

ASJC Scopus subject areas

  • General Chemical Engineering
  • General Chemistry

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