Digital holography for second harmonic microscopy: Application to 3D-tracking of nanoparticles

Etienne Shaffer*, Christian Depeursinge

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations


Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.

Original languageEnglish (US)
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care II
StatePublished - 2010
Externally publishedYes
EventBiophotonics: Photonic Solutions for Better Health Care II - Brussels, Belgium
Duration: Apr 12 2010Apr 16 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherBiophotonics: Photonic Solutions for Better Health Care II


  • 3D-tracking
  • Digital holographic microscopy (DHM)
  • Digital holography
  • Nanoparticles
  • Nonlinear microscopy
  • Second harmonic generation (SHG)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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