TY - GEN
T1 - Digital holographic microscopy for the study of morphological changes in cells under simulated microgravity condition
AU - Pache, C.
AU - Westphal, K.
AU - Parent, J.
AU - Franco-Obregon, A.
AU - Depeursinge, Christian
AU - Egli, M.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Previous investigations on mammalian cells have shown that real microgravity in space and/or simulated microgravity causes severe cellular modifications. Aim of the study was to use Digital Holographic Microscopy (DHM) to investigate morphological changes of cells under simulated microgravity generated by the Random Positioning Machine (RPM) in real-time. Preliminary studies were carried out on mouse myoblasts (C2C12). Besides showing cellular modification under simulated microgravity similar to the results obtained by using conventional methods, an increase of the phase height of the cell was measured which have not been reported yet. Our technique provides a new approach in the study of cellular adaptations to microgravity, providing real-time images of living cells.
AB - Previous investigations on mammalian cells have shown that real microgravity in space and/or simulated microgravity causes severe cellular modifications. Aim of the study was to use Digital Holographic Microscopy (DHM) to investigate morphological changes of cells under simulated microgravity generated by the Random Positioning Machine (RPM) in real-time. Preliminary studies were carried out on mouse myoblasts (C2C12). Besides showing cellular modification under simulated microgravity similar to the results obtained by using conventional methods, an increase of the phase height of the cell was measured which have not been reported yet. Our technique provides a new approach in the study of cellular adaptations to microgravity, providing real-time images of living cells.
UR - http://www.scopus.com/inward/record.url?scp=66949158664&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:66949158664
SN - 9789292212278
T3 - European Space Agency, (Special Publication) ESA SP
BT - Proceedings of the Symposium Life in Space for Life on Earth
PB - European Space Agency
T2 - Life in Space for Life on Earth
Y2 - 22 June 2008 through 27 July 2008
ER -