Abstract
Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate non-invasively cellular structures and dynamics. DHM images of living cells in culture are presented. They represent the distribution of the optical path length over the cell, which contains information concerning both the cellular morphometry and the intracellular refractive index, and which has been measured with a sub-wavelength accuracy.
| Original language | English (US) |
|---|---|
| Article number | 58640C |
| Pages (from-to) | 1-4 |
| Number of pages | 4 |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 5864 |
| State | Published - 2005 |
| Externally published | Yes |
| Event | Novel Optical Instrumentation for Biomedical Applications II - Munich, Germany Duration: Jun 12 2005 → Jun 16 2005 |
Keywords
- Cell analysis
- Coherence imaging
- Digital holographic microscopy
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging